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Validate cosmetic products with multi-omics and clinical testing. Generate evidence, understand mechanisms, and support advanced claims. Advanced claims, backed by biology. SKIN | SCALP | INTIMATE | ORAL We help innovative dermocosmetic and personal care brands generate clinical-grade evidence and multi-omics insight, revealing not just efficacy, but true mechanisms of action and competitive positioning. Prove your product’s impact We go beyond validation, into biological understanding Sequential combines clinical testing with multi-omics and molecular analysis to uncover how products interact with human biology. We don’t just tell you if your product works. We show you: What biological systems it impacts (microbiome, barrier, inflammation, pigmentation, aging) How it works at a molecular level How it compares to other products in your category The era of generic claims is ending. “Clinically tested.” “Dermatologist approved.” “Microbiome-friendly.” These claims are everywhere, and increasingly meaningless. Today’s consumers, regulators, and partners expect more than surface-level validation. They want to understand how a product works, not just what it claims to do. For truly innovative brands, this creates a challenge: How do you stand out when weak claims are easy, and real science is hard to communicate? Clinical-grade evidence demonstrating real biological impact. Credible scientific proof Mechanism of action clarity Translate molecular data into a defensible scientific claim and story. Competitive positioning Understand how your product differentiates and competes within its category. Our process is designed to turn data into clear, defensible claims. We work closely with each client to design tailored clinical studies, recruit relevant participants, and collect high-quality samples using non-invasive methods. Through multi-omics analysis and in-house expertise, we generate a comprehensive understanding of product impact, translating complex biological data into scientifically grounded, claim-ready insights that drive confidence and growth. Start your validation study How it works

< Back Sales Associate (NYC Based) New York, NY, USA Job Type Full-time Workspace Hybrid About the Role As a Sales Associate at Sequential, you will be essential in building relationships and expanding our reach within the personal care and dermocosmetic industry. Your role will involve supporting business development efforts, assisting in sales strategy execution, and helping grow market share for Sequential’s innovative products and services. You will work closely with the Commercial Director and team to meet sales goals, develop client relationships, and contribute to Sequential’s mission of advancing microbiome science. This is a fantastic opportunity for an ambitious sales professional to gain hands-on experience and make a tangible impact in a rapidly growing biotech field. Essential Duties and Responsibilities: Sales Support and Development: Assist in the identification and acquisition of new customer accounts, supporting the growth of Sequential’s client base. Client Relationship Building: Cultivate relationships with existing and prospective clients, focusing on understanding their needs and promoting Sequential’s unique offerings. Market Research: Conduct ongoing research to stay informed on industry trends and competitor activity, providing valuable insights for sales strategies. CRM and Data Management: Maintain accurate records in our CRM (HubSpot), tracking interactions, progress, and key performance indicators. Sales Target Achievement: Work towards defined sales goals, contributing to Sequential’s revenue targets and growth ambitions. Collaborative Efforts: Work closely with marketing, product, and operations teams to align efforts and ensure a seamless customer experience. Product Knowledge Development: Stay up-to-date on Sequential’s product offerings and the latest microbiome research to effectively communicate value to clients. Qualifications and Experience: 1-2 years of sales experience, ideally within the biotechnology, cosmetics, or life sciences fields. Familiarity with CRM systems, preferably HubSpot, and the ability to manage data with accuracy. Strong written and verbal communication skills with a passion for client interaction and customer service. Tech-savvy and eager to leverage technology to enhance sales effectiveness. Bachelor's degree in Life Sciences, Business, or a related field preferred; demonstrated interest in microbiome or biotechnology is a plus. Motivated self-starter with a goal-oriented mindset and excellent organizational skills. Ability to work collaboratively and adapt in a dynamic, fast-paced environment. What You Get from Us: A base salary with a commission structure that rewards your hard work and success. Access to our Equity Incentive Plan, allowing you to grow alongside Sequential as we expand. The chance to work with industry experts in one of the fastest-growing sectors within biotechnology. Be part of a company at the forefront of microbiome research, making an impact in the personal care and dermocosmetic fields. About the Company Sequential is a global leader in the skin microbiome field, a team of PhD experts in testing products and their effect on the human microbiome (skin, scalp, oral, vulva). An Innovate UK- and Enterprise Singapore-backed company, with labs in London, New York City and Singapore. Awarded the title "Most Significant" Testing Solution in the Industry - (C&T, 2022). To date, Sequential has amassed over 25,000 human skin microbiome samples and corresponding formulations tested in vivo on the skin. With this vast genomic dataset, Sequential are figuring out optimal formulations that could potentially alleviate skin conditions, for example – acne, atopic dermatitis and rosacea. They have been supported by Innovate UK, Enterprise SG, A*STAR (Genome Institute of Singapore). They have raised $5MM USD to date from SOSV, Metaplanet, Scrum Ventures, Corundum Systems Biology and are a resident company of JLABS Innovation (in NYC). Apply Now

What Disrupts the Skin Microbiome? What Disrupts the Skin Microbiome? The skin microbiome is an intricate ecosystem of bacteria, fungi, and viruses that protect and maintain skin health (Smythe & Wilkinson, 2023). It acts as a defence against harmful pathogens, regulates inflammation, and supports the skin’s overall barrier function. However, disruptions to this microbiome can eventually lead to skin issues like acne, eczema, and various skin infections (Wallen-Russell, 2019). Understanding these disruptions is key to making informed choices about skincare, diet, and lifestyle to promote healthy skin. 1. Harsh Skincare Products Personal care products like soaps and lotions can disrupt the skin microbiome by removing natural oils and beneficial microbes. Many contain harsh chemicals, such as preservatives and fragrances, which reduce microbial diversity and promote the growth of harmful bacteria like Staphylococcus aureus. A study by Wallen-Russel (2018) found that synthetic ingredients generally lower the positive effects on skin biodiversity. Using essential, pH-balanced skincare products is recommended to maintain a healthy skin microbiome. 2. Antibiotics and Medications The use of antibiotics is a major disruptor of the skin microbiome. While antibiotics are essential for treating bacterial infections, their overuse or misuse can eliminate beneficial bacteria alongside harmful pathogens. This disruption can lead to a decrease in microbial diversity and the dominance of antibiotic-resistant bacteria, which may contribute to skin conditions like eczema and psoriasis. Studies have shown that prolonged antibiotic use can have lasting effects on the skin's microbial communities, making it more prone to dysbiosis and related diseases (Byrd et al., 2018). 3. Environmental Factors Environmental exposures are a major cause of skin microbiome disruption. Pollution, particularly airborne particulate matter and toxins, weakens the skin barrier and alters microbial diversity by causing oxidative stress and inflammation. This imbalance favours harmful microbes while reducing beneficial ones. Araviiskaia et al. (2019) found that chronic inflammatory skin conditions like eczema and psoriasis tend to worsen in individuals, including children, when exposed to high pollution levels. While moderate sun exposure is beneficial, excessive UV exposure can cause acute and chronic skin damage, including inflammation, premature ageing, and increased cancer risk. Patra, Sérézal & Wolf (2020) highlights how UV radiation disrupts the skin microbiome, potentially leading to dysbiosis and compromised skin health. 4. Diet and Lifestyle Diet and lifestyle choices can also direct the overall health of the skin microbiome. Ghosh, McMahon & Lappin (2021) revealed that a plant-based diet can positively influence the skin microbiome, reducing inflammation and oxidative stress, thereby supporting overall skin health. Conversely, diets high in processed foods and saturated fats can negatively impact the microbiome and lead to health issues, including skin conditions. 5. Stress and Hormonal Changes Stress can significantly impact the skin microbiome, primarily through hormonal and behavioural changes. Stress activates the hypothalamic-pituitary-adrenal (HPA) axis, leading to increased production of cortisol and other stress hormones. This hormonal response can cause inflammation and immune dysregulation, which may exacerbate various skin conditions like psoriasis, eczema, and acne. Additionally, stress can lead to changes in behaviour, such as neglecting skincare routines or engaging in unhealthy habits (e.g., smoking, poor diet) that further disrupt the skin microbiome (Holmes et al., 2015). Reference Araviiskaia, E., Berardesca, E., Bieber, T., Gontijo, G., Sanchez Viera, M., Marrot, L., Chuberre, B., & Dreno, B. (2019). The impact of airborne pollution on skin. Journal of the European Academy of Dermatology and Venereology : JEADV, 33(8), 1496–1505. https://doi.org/10.1111/jdv.15583 Byrd, A. L., Belkaid, Y., & Segre, J. A. (2018). The human skin microbiome. Nature Reviews Microbiology, 16(3), 143-155. https://doi.org/10.1038/nrmicro.2017.157 Ghosh, S., McMahon, A., & Lappin, D. F. (2021). The relationship between diet, gut microbiota, and skin health. Nutrients, 13(5), 1568. Holmes, C. J., Plichta, J. K., Gamelli, R. L., & Radek, K. A. (2015). Dynamic Role of Host Stress Responses in Modulating the Cutaneous Microbiome: Implications for Wound Healing and Infection. Advances in wound care, 4(1), 24–37. https://doi.org/10.1089/wound.2014.0546 Patra, V., Sérézal, I. G., & Wolf, P. (2020). Potential of Skin Microbiome, Pro- and/or Pre-Biotics to Affect Local Cutaneous Responses to UV Exposure. Nutrients, 12(6), 1795. https://doi.org/10.3390/nu12061795 Smythe, P., & Wilkinson, H. N. (2023). The skin microbiome: Current landscape and future opportunities. International Journal of Molecular Sciences, 24(4), 3950. https://doi.org/10.3390/ijms24043950 Wallen-Russell, C. (2018). The role of Every-Day Cosmetics in Altering the skin Microbiome: A study using biodiversity. Cosmetics, 6(1), 2. https://doi.org/10.3390/cosmetics6010002 Wallen-Russell, C. (2019). The impact of skin care products on skin chemistry and microbiome dynamics. BMC Biology, 17(1), 47. https://doi.org/10.1186/s12915-019-0660-6

Sequential is honoured to be spoken and written about in numerous publications. We are always keen to speak about the great milestones we are hitting with our personal care microbiome testing platform. Sequential in the Press Men’s grooming shifts toward science-driven solutions 24-Feb-2026 Read Now Sequential: Testing shows skin microbiome plays a role in hyperpigmentation 7-Oct-2025 Read Now The bidirectional skin-gut microbiome: a game-changer for cosmetic formulators 29-Jul-2025 Read Now Sequential and AMILI Secure Prestigious $1.8M UK-Singapore Collaborative R&D Project 13-Feb-2025 Read Now Sequential Launches Industry-First Microbiome Target Panels for Skin Conditions 16-Oct-2024 Read Now From the experts: Top 3 trends in skin microbiome research 20-Jun-2024 Read Now Is Home-Based Skin Microbiome Testing Transforming Beauty and Health Care Industry? 7-Dec-2023 Read Now “Hormone-led changes cause differences to the vaginal ecology that can have an effect on women’s health later in life.” CEO of Sequential 13-Sep-2023 Read Now Sequential and Johnson & Johnson enter partnership to develop new genomic-based skin test 16-Mar-2023 Read Now Sequential Announces New Collaboration Agreement to Quantify Skin Health 15-Mar-2023 Read Now ‘Natural evolution’: Gallinée unveils at-home skin microbiome test kits with Sequential Skin 09-Mar-2023 Read Now Here They Are: Congratulations to the Winners of the 2022 C&T Allēs 29-Sep-2022 Read Now Sequential Skin: Why We Invested 11-Apr-2022 Read Now Sequential Skin Debuts Sequential Bio Testing Solution 09-Mar-2022 Read Now Biotech Start-Up Sequential Skin Raises Funds 10-Dec-2021 Read Now Biotech startup Sequential Skin raises €1.4m – ‘We want to accelerate the future of skin health,’ says CEO 11-Oct-2021 Read Now PODCAST – Holistic view on skin: Sequential Skin on why microbiome testing is crucial to personalised beauty 21-Aug-2020 Read Now Beauté personnalisée: Sequential Skin la Beauty Tech made in Singapore 07-Jul-2020 Read Now GWS 2019 DAY 2 Beauty, Science & Technology: Skin in the Game 25-Nov-2019 Read Now Science-led minimalism shapes future sustainable skin care 13-Jan-2026 Read Now Beautiful Bacteria: Are We On The Cusp Of Microbiome Mainstreaming? 28-Sep-2025 Read Now Sequential Skin and AMILI Secure $1.8M UK-Singapore Grant to Advance Gut-Skin Microbiome Science 14-Feb-2025 Read Now 50694 Gut microbiome differences between patients with moderate to severe Chronic Hand Eczema and healthy subjects Sep-2024 Read Now Is Microbiome Certification even Necessary? 29-Jul-2024 Read Now ‘It’s a really delicate system’: Research on vaginal microbiome shows how little we know about femcare 22-Jan-2024 Read Now Gallinée lance le premier test de santé de la peau et du microbiome à réaliser chez soi 10-Oct-2023 Read Now Lysine-dendrimer: a gentle yet effective acne treatment that maintains skin microbiome, says study 18-Aug-2023 Read Now Acne and ageing: J&J exploring skin health markers via Sequential Skin research tie-up 15-Mar-2023 Read Now Sequential Skin x Johnson & Johnson Collab to Quantify Skin Health 14-Mar-2023 Read Now Understand before purchase: Microbiome testing can help consumers find the right products and eliminate waste 30-Jan-2023 Read Now Sequential Wins New Grant To Propel Skin Microbiome Research 30-Jun-2022 Read Now Sequential Skin launches test to assess ingredient effect on microbiome 22-Mar-2022 Read Now Microbiome start-up Sequential Bio debuts first in-vivo testing for personal care industry 04-Mar-2022 Read Now Sequential Skin Raises $1.6M in Oversubscribed Seed Round 12-Oct-2021 Read Now The skin microbiome brainstorm: Tech, claims and trend-driven NPD 16-Sep-2021 Read Now Skin Sensors Enable Personalized Skin Care 20-Aug-2020 Read Now Sequential Skin: beauty tech’s new approach to genetic analysis and microbiome skincare 09-Mar-2020 Read Now How genetic insights can personalise skin microbiome launches 29-Jul-2019 Read Now Deep dive into Radiance Evaluation 9-Oct-2025 Read Now Men’s care shifts toward function-first to address skin needs beyond the basics 16-Sep-2025 Read Now Sequential and AMILI secure $1.8M UK-Singapore R&D project to advance gut-skin microbiome science 13-Feb-2025 Read Now New clinical testing to “investigate microbial roots of various skin conditions” 17-Oct-2024 Read Now Losing its meaning? ‘Microbiome-friendly’ terms at risk of misleading consumers – Sequential CEO 29-Jul-2024 Read Now Navigating the realm of intimate care products 17-Jan-2024 Read Now Avec ce test microbiome maison, 10 secondes m'ont suffit pour découvrir les besoins de ma peau 04-Oct-2023 Read Now Lysine-Dendrimer, a New Non-Aggressive Solution to Rebalance the Microbiota of Acne-Prone Skin 3-Aug-2023 Read Now Sequential Announces New Collaboration Agreement to Measure Skin Health for Leading Developer of Personal Care Products 15-Mar-2023 Read Now Sequential Skin partners with Johnson & Johnson on microbiome tests 10-Mar-2023 Read Now Symbiome Partners with Skin Microbiome Testing experts at Sequential to Revolutionize Beauty Industry 26-Jan-2023 Read Now Sequential Skin Nabs $500K UK Grant to Study Skin Microbiome in Atopic Dermatitis 29-Jun-2022 Read Now Demands rising for clinical data on the skin microbiome: Experts 22-Mar-2022 Read Now ‘Maintaining the microbiome’: Biotech startup Sequential Skin debuts in vivo B2B testing service 03-Mar-2022 Read Now Start-up Sequential Skin bags $1.65m of fresh funding 12-Oct-2021 Read Now Singapore Beauty Startup Sequential Skin Uses Science to Help Identify the Best Skincare For You 07-Jan-2021 Read Now Overlooked opportunities? Skin microbiome may play a more important role in personalisation than DNA 13-Aug-2020 Read Now Sequential tool kit prioritises personalisation 15-Jan-2020 Read Now

Elevate your brand with skin microbiome science. Gain a competitive edge with certified, innovative personal care products rooted in robust research. in vivo Sampling is the Future of Microbiome Testing We lead in clinical microbiome research and testing offering a comprehensive end-to-end platform designed to bring science-backed solutions to the personal care and pharma industry. We offer an expansive platform to conduct research on personal care products through microbiome testing, biophysical assessments, and offer full recruitment services for studies. We are keen to publish our findings with our partners to increase the literature within this space. At present our database of over 20,000 human microbiome samples is one of the most sophisticated within the industry and is growing rapidly. Skin Test your skincare products, ranging from facial cleansers, serums, moisturisers, and SPFs. Scalp Test your scalp care products ranging from scalp serums, oils, shampoos, and conditioners. Vaginal Test your intimate care products, ranging from washes, to hygiene sprays, and balms. Oral Test your oral care products ranging from mouth washes and toothpastes. State of the Art Sequencing Using next-generation sequencing of the collection of micro-organisms found on the body, during product usage, Sequential investigates the microbial diversity, and particular micro-organisms we know are important and play a role in a healthy microbiome. With your pick from one of four sequencing reports, Sequential makes it easier to understand what depth of analysis is required for your particular needs. qPCR Microbiome (Bacteria+Fungi) Quantitative PCR (qPCR), also known as Real-Time PCR, is a method that Sequential uses in-house to measure the absolute number of copies of a microbial DNA defined by Sequential’s proprietary Smart Probes™. Using this method, we quantify the amount bacteria or fungi, allowing us to report level of species and strains within a given sample. The entire process is done at Sequential to ensure quality control, and extra control on your samples and privacy. Up Next-Generation Sequencing of ITS (total fungal population) ITS rRNA gene sequencing, or ITS amplicon sequencing is performed at Sequential to determine the relative abundance of taxa in a fungal community, and to compare between groups of interest. We know that fungi play a critical role in the microbiome of a healthy human, and it’s important to consider this application, especially for the scalp microbiome, and vaginal or vulva microbiome. We routinely offer two types of ITS rRNA primer regions: ITS1 & ITS2 Up Next-Generation Sequencing of 16S 16S rRNA gene sequencing, or 16S amplicon sequencing, is performed at Sequential to determine the relative abundance of taxa in a bacterial community, and to compare between groups of interest. This level of analysis can help to address changes in the overall bacterial profile over time or between treatment groups. We routinely offer two types of 16s rRNA primer regions: 16S V4 & 16S V1-V3 Up Shotgun Metagenomic Sequencing Shotgun metagenome sequencing is performed at Sequential for taxonomic profiling (diversity and abundance), as well as functional analysis across different organisms in a more exploratory approach. This complex technique allows for parallel sequencing of DNA from all organisms (bacterial, fungal, viral and human) within the community, with good coverage for species-level detection. The data generation allows for more advanced reporting and genome assemblies. Up FAQ What is Sequential's testing platform? Sequential has developed the gold standard test for microbiome-friendly products, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. We offer a complete end-to-end solution to support microbiome-friendly claims. From consultancy and study design to our proprietary microbiome testing kits. We analyse, interpret and report our findings to meet your needs. Why is it necessary to test the microbiome in vivo? At present, there are no regulations for microbiome-related formulas that brands and formulators can follow, however, it has been universally acknowledged that the in vivo method of conducting clinical studies is becoming critical and paramount to getting marketing claims through. When regulations are introduced, which may be imminent, the in vitro system will find itself lacking, resulting in limited claims and certifications that do not hold their value. This is why, we at Sequential strive to offer an in vivo approach, knowing full well that we want our client's claims to be significantly backed by scientific and quantifiable data. What type of sequencing technology does Sequential use for analysis? We offer four types of sequencing techniques including qPCR with our Smart Probes™, 16S, ITS and Shotgun Metagenomics. Using next-generation sequencing of the collection of microorganisms found on the body, during product usage, Sequential investigates the microbial diversity, and particular microorganisms we know are important and play a role in a healthy microbiome. Does Sequential offer claims certification for tested products? We provide our clients with a certification to claim “Maintains the Microbiome” subject to in vivo testing results which can be used in communication efforts. Once your product is tested with our qPCR Smart Probes™ and has shown favourable results in supporting the microbiome, we can certify your product with our Maintains the Microbiome certification seal. We have ensured that our seal and certification are backed by quantifiable data and scientifically significant markers. The aim is to ensure our clients feel confident in making their claims and can communicate the true benefit of their microbiome formulations.

Rosacea is a chronic skin condition affecting an estimated 16 million Americans and millions more worldwide, primarily adults over 30 (National Rosacea Society). TARGETED SKIN PANEL Rosacea Targeted Panel 23 Smart Probes ™ Rosacea Panel Validated targets: Demodex mites, Bacillus oleronius, Corynebacterium kroppenstedtii Absolute Quantification End-to-end Service Qet a Quote Unlock the Secrets of Rosacea with Sequential's Cutting-Edge Microbiome Testing Why Microbiome Testing for Rosacea? Rosacea is a chronic skin condition affecting an estimated 16 million Americans and millions more worldwide, primarily adults over 30 (National Rosacea Society). Characterized by redness, visible blood vessels, and inflammation, rosacea can significantly impact an individual’s quality of life. Research suggests that the skin microbiome plays a crucial role in the development and severity of rosacea. Specifically, imbalances in microbial populations, such as an overgrowth of Demodex mites or harmful bacteria, can exacerbate symptoms. In vivo testing of skincare products for rosacea is essential to ensure that formulations are compatible with the unique microbiome of rosacea-prone skin. Such testing allows researchers to observe real-world effects on microbial balance and inflammation, ensuring that products do not unintentionally worsen symptoms. Without this careful testing, products may disrupt the microbiome further, leading to increased redness, irritation, or flare-ups. Sequential is leading the way in microbiome testing for the industry. With a specialized focus on skin conditions, we have developed an innovative solution tailored to help you understand the root causes of rosacea at the microbial level. Our proprietary sequencing panel is designed specifically to offer in-depth analysis of rosacea-associated microbiota, giving you the tools to transform skincare solutions. Customized Analysis Tailored insights specific to rosacea-prone skin, allowing your R&D team to develop more personalized and effective products. Actionable Data Leverage detailed reports to guide formulation decisions and validate product efficacy with precision. Product Innovation New rosacea treatments or enhancing existing lines, our data helps you create targeted solutions with proven results. Precision Sequencing Target specific microbial communities associated with rosacea development and severity. Smart Probe s ™ Our dedicated team of scientists has developed a revolutionary method for evaluating microbes through our Smart Probes™, a proprietary panel that includes 23 key microbes specifically identified as having the most significant impact on skin health. To further understand the complex relationship between rosacea and the skin microbiome, we utilize qPCR technology. This advanced method allows us to accurately quantify microbial populations on the skin, offering deeper insights into how rosacea affects the balance of beneficial and harmful microbes. By closely monitoring how skincare products interact with these microbes, companies can develop solutions that not only target rosacea symptoms but also help restore a healthier, balanced skin microbiome, providing long-term relief for sufferers. Rosacea Panel Targets Demodex mites (Demodex folliculorum and Demodex brevis) These tiny mites are normally present on the skin but are often found in higher numbers on the skin of individuals with rosacea. Their presence is thought to trigger inflammatory responses. Forton FMN. The Pathogenic Role of Demodex Mites in Rosacea: A Potential Therapeutic Target Already in Erythematotelangiectatic Rosacea? Dermatol Ther (Heidelb). 2020 Dec;10(6):1229-1253. doi: 10.1007/s13555-020-00458-9. Epub 2020 Oct 23. PMID: 33095403; PMCID: PMC7649190. Kim HS. Microbiota in Rosacea. Am J Clin Dermatol. 2020 Sep;21(Suppl 1):25-35. doi: 10.1007/s40257-020-00546-8. PMID: 32914214; PMCID: PMC7584533. Bacillus oleronius This bacterium has been linked to rosacea, particularly in relation to the inflammatory response associated with the condition. It is often found in higher quantities on the skin of rosacea patients. Corynebacterium kroppenstedtii This bacterium appears to be significantly overrepresented in papulopustular and mixed rosacea compared to healthy skin, with its presence correlating with disease severity and lesion spread, suggesting a potential opportunistic or contributory role in rosacea pathophysiology. Chien AL, Kang S, et al. Characterization and Analysis of the Skin Microbiota in Rosacea. J Am Acad Dermatol. 2020; PMID: 31502207. Industry Leader in Absolute Quantification Sequential’s Smart Probe ™ qPCR approach to microbiome analysis can resolve species and subspecies, giving statistically significant findings between skin conditions on in vivo human samples, compared to conventional sequencing approaches. Relevant Research How Does the Skin Microbiome Influence Rosacea? Unveiling the Microbial Puzzle Read More Articles

Multi-Omic Studies with Sequential At Sequential, our mission has always been clear: to understand the impact of the microbiome on humans and how humans impact the microbiome in order to characterize human health fully. Our Multi-Omic Studies brings us back to the core of our mission by offering an unparalleled approach to skin health research. By combining human and microbial analysis, Sequential is setting a new standard in clinical testing. This comprehensive methodology enables us to unlock deeper insights into the complex interplay between the microbiome and human biology, offering more tailored and effective product assessments for our partners in personal care and the pharmaceutical industries. Start Your Multi-Omic Study Today! *Hover over the diagram to unlock each area that we explore! Identify key genetic markers linked to health, aging, and disease susceptibility Predict responsiveness to product SNP Detection & Risk Assessment Gene Expression Profile Uncover how genes are activated in response to lifestyle, environment, and treatments The effect of product on skin-related gene expression Protein Profiling Analyse protein biomarkers to track biological changes and optimise product efficacy Measure skin protein production after a product treatment Lipid Profiling Investigate lipid composition for insights into skin barrier function and metabolic health Assess how a product restores lipid homeostasis qPCR Sequencing Investigate the absolute quantification of key microbes in any sample Assess how a product impacts the most significant bacteria in a sample Metatranscriptomics Uncover how genes are activated in response to lifestyle, environment, & treatments. The effect of product on skin-related microbial gene expression ITS Sequencing Investigate all present fungi in any given sample Understand how a product impacts the skin's fungal community Shotgun Metagenomics Investigate all present microorganisms in any given sample Understand how a product impacts the skin's bacteria, fungal and viral communities 16S Sequencing Investigate all present bacteria in any given sample Understand how a product impacts the skin's bacterial community Metabolomics Map metabolic pathways to assess skin and body health, aging, and personalized wellness. Metabolic shift pre- and post-product application Why Multi-Omic Testing? Understanding how products impact both the human body and its microbiome is essential for creating truly effective solutions. Our multi-omic approach provides: Deeper Insights – See how products influence human health at a biological level Clinically Validated Science – Data-backed results to support claims and innovation Personalized Health & Wellness – Create tailored products that optimize skin and body health Scalable & Flexible Testing – In-lab or remote studies with diverse sample collection options Microbiome's Impact on Human Health The Bright Side of Skincare: How LEDs Influence Microbial Balance With LED face masks and light-based skincare rapidly increasing in popularity, light has become a new frontier in skin health. Previously seen as a threat and something that we needed to block or avoid, light is now being seen as therapeutic tool. Yet beyond the visible changes to tone and texture, light also interacts with the skin microbiome. Understanding how different wavelengths influence this ecosystem is key to ensuring that light enhances, rather than harms, our skin’ How Pregnancy and Postpartum Transform the Skin Microbiome During pregnancy, the skin microbiome undergoes significant shifts. Across the trimesters, both the composition and behaviour of skin bacteria change. Postpartum, the maternal skin microbiome continues to evolve which is influenced by hormonal resets, environmental exposures, and hygiene practices. These shifts can also influence an infant’s earliest microbial encounters, during a critical period of skin development. What We Know: Pregnancy significantly changes the skin mic Understanding the Microbial Roots of Seborrheic Dermatitis Seborrheic dermatitis (SD) is a common inflammatory skin condition primarily affecting the scalp, face and chest. It appears as red, flaky, greasy patches or plaques and can cause itching and discomfort. It is a non-contagious condition varying in severity, from mild dandruff to persistent inflamed lesions. What we know: SD is linked to three main factors: increased sebum (oil) production, colonization by Malassezia yeast, and an abnormal immune response. Genetics, skin barr Your Muti-Omics Questions Answered What is multi-omic testing, and why is it important? Multi-omic testing integrates multiple layers of biological data—including genomics, metabolomics, proteomics, and microbiome science—to provide a more comprehensive understanding of how products impact both the human body and its microbiome. This approach goes beyond traditional microbiome testing, helping brands develop more effective, scientifically backed solutions. How does Sequential’s multi-omic approach differ from traditional microbiome testing? Most microbiome studies focus only on bacterial composition. At Sequential, we go further by analyzing: Human gene expression to understand how a product influences skin health at the molecular level Metabolomics & lipid profiling to track changes in skin health and barrier function Protein profiling to measure product-driven biological changes Advanced microbiome techniques to assess bacteria, fungi, and viruses collectively This holistic approach ensures deeper insights for product development and claim validation. What can multi-omic testing help me understand about my product? Product effectiveness – See how formulations impact skin and microbiome health Personalized wellness – Identify genetic or metabolic factors that influence individual responses to products Barrier function & hydration – Understand how products restore lipid balance and improve the skin barrier Microbial balance – Determine whether a product supports a healthy microbiome or disrupts it What specific tests does Sequential offer under multi-omic analysis? Human Health Insights SNP Detection & Risk Assessment – Identifies genetic markers related to aging, disease risk, and product responsiveness Metabolomics – Maps metabolic pathways to track skin and body health before and after product use Human Gene Expression – Determines how genes react to lifestyle, environmental factors, and treatments Protein Profiling – Measures skin protein production to optimize product efficacy Lipid Profiling – Assesses lipid composition for insights into skin barrier health Microbiome Science & Testing Smart Probes™ qPCR – Tracks 20 key microbes to measure microbiome shifts after product use Microbial Profiling (16S & ITS) – Identifies all bacteria and fungi in a sample Microbial Gene Expression (Metatranscriptomics) – Evaluates how microbes react to environmental factors and treatments Shotgun Metagenomics – Provides a complete profile of bacteria, fungi, and viruses in any sample

Sequential is proud to announce that is has been dubbed the most significant testing method in the industry for microbiome testing. Allē Award: Sequential Wins Prestigious “Most Significant” Testing Method After being listed as a finalist as best ‘Claims testing methods & tools’ alongside Evonik, and XCellR8 - Sequential brings home top prize in C&T’s Allē Awards, 2022. The personal care and cosmetics industry in the US is valued at $190B. In the context of the skin microbiome, the industry is still a relatively nascent field. However, its projected growth is significant, estimated to grow at a compounded annual growth of 29.2% between 2022-2028. Owed to the impact of products on the skin microbiome, consumer awareness, and understanding that our microbiome is intimately linked to our health. Sequential is the B2B microbiome testing arm for Sequential Skin Ltd, which has developed the world’s first end-to-end platform for companies to evaluate their products on the microbiome in vivo. They specialize in skin, scalp and intimate area microbiome. "Of all the 'microbiome friendly' methodologies working to address both consumer and industry concerns about the impact of cosmetics on the microbiome, this is, in my opinion, the most quantitative and promising of the lot." – C&T's Allē Award judges, 2022. The company has developed a proprietary non-invasive method for collecting skin samples to analyze the skin microbiome using next-generation sequencing (NGS), with species and strain identification, in longitudinal (and clinical) studies. Services include data interpretation and the results are reported in a comprehensive, yet understandable format, ready for formulators and research scientists to incorporate into their product development pipeline. “The Allē Award gives us strong confidence in the testing platform and capabilities we’ve built in Sequential. Being the first to develop an in vivo microbiome test for the industry, we’re proud to see our hard work paying off” — Petronille Houdart, DPharm, skincare director at Sequential. About Sequential Sequential is part of the Sequential Skin group – with a US lab in New York City, alongside a lab in London and a lab in Singapore. Sequential team has over 20 years of combined expertise in genetics, epigenetics, and microbiome research. Sequential has validated its AI-driven testing platform with over 30 companies, analyzing over 12,000 skin microbiome samples. They specialise in skin, scalp and vulva/vaginal microbiome samples. They are supported by Enterprise SG, A*STAR, Genome Institute of Singapore, IndieBio New York, SOSV, Metaplanet Holdings, Scrum Ventures, Genedant VC, Ben Holmes (ex. General Partner at Index Ventures), Innovate UK, and are a resident company of Johnson & Johnson Innovation – JLABS.

At Sequential we take your privacy seriously. For a full breakdown, please read through our policy on how we use your information for microbiome product testing. Privacy Policy 1. Introduction 1.1 Important information and who we are Welcome to Sequential Skin Ltd’s Privacy and Data Protection Policy (“Privacy Policy”). At Sequential Skin Ltd (“we”, “us”, or “our”) we are committed to protecting and respecting your privacy and Personal Data in compliance with the United Kingdom General Data Protection Regulation (“GDPR”), the Data Protection Act 2018 and all other mandatory laws and regulations of the United Kingdom. This Privacy Policy explains how we collect, process and keep your data safe. The Privacy Policy will tell you about your privacy rights, how the law protects you, and inform our employees and staff members of all their obligations and protocols when processing data. The individuals from which we may gather and use data can include: Customers Suppliers Business contacts Employees/Staff Members Third parties connected to your customers Collaborators, academic institutes, industry partners. and any other people that the organisation has a relationship with or may need to contact. This Privacy Policy applies to all our employees and staff members and all Personal Data processed at any time by us. 1.2 Your Data Controller Sequential Skin Ltd is your Data Controller and responsible for your Personal Data. We are not obliged by the GDPR to appoint a data protection officer and have not voluntarily appointed one at this time. Therefore, any inquiries about your data should either be sent to us by email to team@sequential.bio or by post to 85 Great Portland Street, London, W1W 7LT, United Kingdom. You have the right to make a complaint at any time to the Information Commissioner’s Office (ICO), the UK supervisory authority for data protection issues (www.ico.org.uk ). We would, however, appreciate the chance to deal with your concerns before you approach the ICO so please contact us in the first instance. 1.3 Processing data on behalf of a Controller and processors’ responsibility to you In discharging our responsibilities as a Data Controller we have employees who will deal with your data on our behalf (known as “Processors”). The responsibilities below may be assigned to an individual or may be taken to apply to the organisation as a whole. The Data Controller and our Processors have the following responsibilities: Ensure that all processing of Personal Data is governed by one of the legal bases laid out in the GDPR (see 2.2 below for more information); Ensure that Processors authorised to process Personal Data have committed themselves to confidentiality or are under an appropriate statutory obligation of confidentiality; Implement appropriate technical and organisational measures to ensure a level of security appropriate to the risk associated with the processing of Personal Data; Obtain the prior specific or general authorisation of the Controller before engaging another Processor; Assist the Controller in the fulfilment of the Controller's obligation to respond to requests for exercising the data subject's rights; Make available to the Controller all information necessary to demonstrate compliance with the obligations laid down in the GDPR and allow for and contribute to audits, including inspections, conducted by the Controller or another auditor mandated by the Controller; Maintain a record of all categories of processing activities carried out on behalf of a Controller; Cooperate, on request, with the supervisory authority in the performance of its tasks; Ensure that any person acting under the authority of the Processor who has access to Personal Data does not process Personal Data except on instructions from the Controller; and Notify the Controller without undue delay after becoming aware of a Personal Data Breach. 2. Legal Basis for Data Collection 2.1 Types of data / Privacy policy scope “Personal Data” means any information about an individual from which that person can be identified. It does not include data where the identity has been removed (anonymous data). We may collect, use, store and transfer different kinds of Personal Data about you which we have grouped together below. Not all of the following types of data will necessarily be collected from you but this is the full scope of data that we collect and when we collect it from you: Profile/Identity Data: This is data relating to your first name, last name, gender, date of birth. Contact Data: This is data relating to your phone number, addresses, email addresses, phone numbers. Marketing and Communications Data: This is your preferences in receiving marketing information and other information from us. Technical Data: This is your IP address, browser type and version, time zone setting and location, operating system and platform, and other technology on the devices you use to engage with us. Customer Support Data: This includes feedback and survey responses. Usage Data: information about how you use our website, products and services. We do not collect any Special Categories of Personal Data about you (this includes details about your race or ethnicity, religious or philosophical beliefs, sex life, sexual orientation, political opinions, trade union membership, information about your health, and genetic and biometric data). Nor do we collect any information about criminal convictions and offences. 2.2 The Legal Basis for Collecting That Data There are a number of justifiable reasons under the GDPR that allow collection and processing of Personal Data. The main avenues we rely on are: “Consent”: Certain situations allow us to collect your Personal Data, such as when you tick a box that confirms you are happy to receive email newsletters from us, or ‘opt in’ to a service. “Contractual Obligations”: We may require certain information from you in order to fulfil our contractual obligations and provide you with the promised service. “Legal Compliance”: We’re required by law to collect and process certain types of data, such as fraudulent activity or other illegal actions. “Legitimate Interest”: We might need to collect certain information from you to be able to meet our legitimate interests - this covers aspects that can be reasonably expected as part of running our business, that will not have a material impact on your rights, freedom or interests. Examples could be your address, so that we know where to deliver something to, or your name, so that we have a record of who to contact moving forwards. 3. How We Use your Personal Data 3.1 Our data uses We will only use your Personal Data when the law allows us to. Set out below is a table containing the different types of Personal Data we collect and the lawful basis for processing that data. Please refer to section 2.2 for more information on the lawful basis listed in the table below. Examples provided in the table below are indicative in nature and the purposes for which we use your data may be broader than described but we will never process your data without a legal basis for doing so and it is for a related purpose. For further inquiries please contact us. 3.2 Marketing and content updates You will receive marketing and new content communications from us if you have created an account and chosen to opt into receiving those communications. From time to time we may make suggestions and recommendations to you about goods or services that may be of interest to you. 3.3 Change of purpose We will only use your Personal Data for the purposes for which we collected it, unless we reasonably consider that we need to use it for another reason and that reason is compatible with the original purpose. If you wish to get an explanation as to how the processing for the new purpose is compatible with the original purpose, please contact us. If we need to use your Personal Data for an unrelated purpose, we will notify you and we will explain the legal basis which allows us to do so. Please note that we may process your Personal Data without your knowledge or consent, in compliance with the above rules, where this is required or permitted by law. 4. Your Rights and How You Are Protected By Us 4.1 Your Legal Rights Under certain circumstances, you have the following rights under data protection laws in relation to your personal data: Right to be informed. You have a right to be informed about our purposes for processing your personal data, how long we store it for, and who it will be shared with. We have provided this information to you in this policy. Right of access. This enables you to receive a copy of the personal data we hold about you and to check that we are lawfully processing it (also known as a "data subject access request"). See section 4.4 below for more details on how you can make a data subject access request. Right to rectification. You have a right to request correction of the personal data that we hold about you. This enables you to have any incomplete or inaccurate data we hold about you corrected, though we may need to verify the accuracy of the new data you provide to us. Right to erasure. You have the right to ask us to delete or remove personal data where there is no good reason for us continuing to process it, where you have successfully exercised your right to object to processing (see below), where we may have processed your information unlawfully or where we are required to erase your personal data to comply with local law. Note, however, that we may not always be able to comply with your request of erasure for specific legal reasons which will be notified to you, if applicable, at the time of your request. Right to object. You can object to the processing of personal data we hold about you. This effectively allows you to stop or prevent us from processing your personal data. Note that this is not an absolute right and it only applies in certain circumstances, for example: (i) Where we are processing your personal data for direct marketing purposes. (ii) Where we are relying on a legitimate interest (or those of a third party) and there is something about your particular situation which makes you want to object to processing on this ground as you feel it impacts on your fundamental rights and freedoms. (iii) In some cases, we may continue processing your data if we can demonstrate that we have compelling legitimate grounds to process your information which override your rights and freedoms. Right to restrict processing. You have the right to request the restriction or suppression of their personal data. Note that this is not an absolute right and it only applies in certain circumstances: (i) If you want us to establish the data's accuracy. (ii) Where our use of the data is unlawful but you do not want us to erase it. (iii) Where you need us to hold the data even if we no longer require it as you need it to establish, exercise or defend legal claims. (iv) You have objected to our use of your data but we need to verify whether we have overriding legitimate grounds to use it. Right to data portability. You have the right to request the transfer of your personal data to you or to a third party. If you make such a request, we will provide to you, or a third party you have chosen, your personal data in a structured, commonly used, machine-readable format. Note that this right only applies to automated information which you initially provided consent for us to use or where we used the information to perform a contract with you. If you wish to make a request under any of these rights, please contact us at team@sequential.bio . 4.2 How Sequential Skin Ltd protects customers' Personal Data We are concerned with keeping your data secure and protecting it from inappropriate disclosure. We implement a variety of security measures to ensure the security of your Personal Data on our systems, including We implement certain security measures to ensure the security of your Personal Data on our systems, including using encrypted servers that gives confidence that personal data is being treated securely.. Any Personal Data collected by us is only accessible by a limited number of employees who have special access rights to such systems and are bound by obligations of confidentiality. If and when we use subcontractors to store your data, we will not relinquish control of your Personal Data or expose it to security risks that would not have arisen had the data remained in our possession. However, unfortunately no transmission of data over the internet is guaranteed to be completely secure. It may be possible for third parties not under the control of Sequential Skin Ltd to intercept or access transmissions or private communications unlawfully. While we strive to protect your Personal Data, we cannot ensure or warrant the security of any Personal Data you transmit to us. Any such transmission is done at your own risk. If you believe that your interaction with us is no longer secure, please contact us. 4.3 Opting out of marketing promotions You can ask us to stop sending you marketing messages at any time by unsubscribing at the bottom of the email. Where you opt out of receiving these marketing messages, we will continue to retain other Personal Data provided to us as a result of interactions with us not related to your marketing preferences. 4.4 How to request your data and the process for obtaining it You will not have to pay a fee to access your Personal Data (or to exercise any of the other rights). However, if your request is clearly unfounded, we could refuse to comply with your request. We may need to request specific information from you to help us confirm your identity and ensure you have the right to access your Personal Data (or to exercise any of your other rights). This is a security measure to ensure that Personal Data is not disclosed to any person who has no right to receive it. We may also contact you to ask you for further information in relation to your request to speed up our response. 5. Your Data and Third Parties 5.1 Sharing your data with third parties We may share non-Personal Data with third parties. We may share your Personal Data with subcontractors or affiliates, subject to confidentiality obligations to use it only for the purposes for which we disclose it to them and pursuant to our instructions. We may also share Personal Data with interested parties in the event that Sequential Skin Ltd anticipates a change in control or the acquisition of all or part of our business or assets or with interested parties in connection with the licensing of our technology. If Sequential Skin Ltd is sold or makes a sale or transfer, we may, in our sole discretion, transfer, sell or assign your Personal Data to a third party as part of or in connection with that transaction. Upon such transfer, the Privacy Policy of the acquiring entity may govern the further use of your Personal Data. In all other situations your data will still remain protected in accordance with this Privacy Policy (as amended from time to time). We may share your Personal Data at any time if required for legal reasons or in order to enforce our terms or this Privacy Policy. 5.2 Third-Party Links This Site may include links to third-party websites, plug-ins and applications. Clicking on those links or enabling those connections may allow third parties to collect or share data about you. We do not control these third-party websites and are not responsible for their privacy statements. When you leave our Site, we encourage you to read the privacy policy of every website you visit. 6. How Long We Retain Your information We will only retain your Personal Data for as long as reasonably necessary to fulfil the purposes we collected it for. We may retain your Personal Data for a longer period than usual in the event of a complaint or if we reasonably believe there is a prospect of litigation in respect to our relationship with you. 7. International Transfer of Data Your information may be stored and processed in the US or other countries or jurisdictions outside the US where Sequential Skin Ltd has facilities. By using Sequential Skin Ltd, you are permitting and consenting to the transfer of information, including Personal Data, outside of the US. 8. Notification of Changes and Acceptance of Policy We keep our Privacy Policy under review and will place any updates here. This version is dated 23 February 2024. By using Sequential Skin Ltd, you consent to the collection and use of data by us as set out in this Privacy Policy. Continued access or use of Sequential Skin Ltd will constitute your express acceptance of any modifications to this Privacy Policy. 9. Interpretation All uses of the word "including" mean "including but not limited to" and the enumerated examples are not intended to in any way limit the term which they serve to illustrate. Any email addresses set out in this policy may be used solely for the purpose for which they are stated to be provided, and any unrelated correspondence will be ignored. Unless otherwise required by law, we reserve the right to not respond to emails, even if they relate to a legitimate subject matter for which we have provided an email address. You are more likely to get a reply if your request or question is polite, reasonable and there is no relatively obvious other way to deal with or answer your concern or question (e.g. FAQs, other areas of our website, etc.). Our staff are not authorised to contract on behalf of Sequential Skin Ltd, waive rights or make representations (whether contractual or otherwise). If anything contained in an email from a Sequential Skin Ltd address contradicts anything in this policy, our terms or any official public announcement on our website, or is inconsistent with or amounts to a waiver of any Sequential Skin Ltd rights, the email content will be read down to grant precedence to the latter. The only exception to this is genuine correspondence expressed to be from the Sequential Skin Ltd legal department.

Video on how to take a microbiome sample with Sequential's microbiome testing kit.

Malodor, or body odor, affects millions of people globally, with studies suggesting that up to 90% of individuals experience some form of body odor during their lifetime, especially in areas such as the underarms, feet, and groin (American Society for Microbiology). TARGETED SKIN PANEL Malodor Targeted Panel 23 Smart Probes ™ Malodor Panel Validated targets: Corynebacterium species, Staphylococcus hominis, Cutibactrium acnes species Absolute Quantification End-to-end Service Qet a Quote Unlock the Secrets of Malodor with Sequential's Cutting-Edge Microbiome Testing Why Microbiome Testing for Malodor? Malodor, or body odor, affects millions of people globally, with studies suggesting that up to 90% of individuals experience some form of body odor during their lifetime, especially in areas such as the underarms, feet, and groin (American Society for Microbiology). This issue arises primarily due to interactions between sweat and the skin’s microbiome, where certain bacteria, like Corynebacterium and Staphylococcus , break down sweat molecules into odorous compounds. The composition of a person’s microbiome greatly influences the severity and type of body odor they experience, which highlights the need for microbiome-focused deodorant formulations. In vivo testing is crucial for deodorants because it allows scientists to see how the product affects both sweat production and the microbiome in real-world conditions. Without proper testing, deodorants might mask odors temporarily but disrupt the natural microbial balance, leading to skin irritation or increased malodour over time. By ensuring deodorants maintain a healthy microbiome, brands can create more effective, long-lasting solutions for body odor. Sequential is leading the way in microbiome testing for the industry. With a specialized focus on skin conditions, we have developed an innovative solution tailored to help you understand the root causes of malodor at the microbial level. Our proprietary sequencing panel is designed specifically to offer in-depth analysis of malodor-associated microbiota, giving you the tools to transform skincare solutions. Customized Analysis Tailored insights specific to malodor, allowing your R&D team to develop more personalized and effective products. Actionable Data Leverage detailed reports to guide formulation decisions and validate product efficacy with precision. Product Innovation New deoderants or enhancing existing lines, our data helps you create targeted solutions with proven results. Precision Sequencing Target specific microbial communities associated with malodor. Smart Probe s ™ Our dedicated team of scientists has developed a groundbreaking method for evaluating microbes through our Smart Probes™, a proprietary panel featuring 23 key microbes specifically identified as having the greatest impact on skin health, particularly in relation to malodor. To further investigate the complex relationship between body odor and the skin microbiome, we employ qPCR technology. This advanced technique enables us to precisely measure microbial populations on the skin, providing deeper insights into how imbalances in the microbiome contribute to malodor, such as the activity of bacteria like Corynebacterium and Staphylococcus . By carefully analyzing how deodorant and skincare products interact with odor-causing microbes, companies can develop solutions that not only target and reduce malodor but also support a healthy, balanced skin microbiome, ensuring long-lasting freshness and overall skin health. Malodor Panel Targets Corynebacterium species These bacteria are known to break down sweat into fatty acids and other compounds that contribute to body odor. They are particularly prevalent in areas like the armpits. Ågren MS, Ghathian KSA, Frederiksen AKS, Bjerrum MJ, Calum H, Danielsen PL, Menon J, Hædersdal M, Jorgensen LN. Zinc Oxide Inhibits Axillary Colonization by Members of the Genus Corynebacterium and Attenuates Self-perceived Malodour: A Randomized, Double-blind, Placebo-controlled Trial. Acta Derm Venereol. 2020 May 28;100(10):adv00145. doi: 10.2340/00015555-3499. PMID: 32399578; PMCID: PMC9137363. Staphylococcus hominis This bacterium is involved in the breakdown of sweat and the production of odor-causing compounds. It is commonly found in areas with high sweat gland activity. Lam, T.H., Verzotto, D., Brahma, P. et al. Understanding the microbial basis of body odor in pre-pubescent children and teenagers. Microbiome 6, 213 (2018). https://doi.org/10.1186/s40168-018-0588-z Cutibacterium acnes species Although better known for its role in acne, this bacterium also contributes to body odor by metabolizing sweat and producing odoriferous substances. Coenye T, Spittaels KJ, Achermann Y. The role of biofilm formation in the pathogenesis and antimicrobial susceptibility of Cutibacteriumacnes. Biofilm. 2021 Dec 9;4:100063. doi: 10.1016/j.bioflm.2021.100063. PMID: 34950868; PMCID: PMC8671523. Industry Leader in Absolute Quantification Sequential’s Smart Probe ™ qPCR approach to microbiome analysis can resolve species and subspecies, giving statistically significant findings between skin conditions on in vivo human samples, compared to conventional sequencing approaches. Relevant Research Unveiling the Microbial Truth: The Unpleasant Reality of Scalp Malodour Don't Sweat It: How Deodorant Disrupts Your Underarm Microbiome Read More Articles

How Do I Test My Microbiome? How Do I Test My Microbiome? The microbiome refers to the collection of trillions of microorganisms, such as bacteria, fungi, and viruses, that live in and on your body, particularly in your gut. (Lloyd-Price, Abu-Ali & Huttenhower, 2016) These microbes are vital components in maintaining our health by aiding digestion, regulating the immune system, and even influencing mood and overall brain function. Given the growing interest in the microbiome's impact on overall well-being, testing the microbiome has broadened its appeal among people. Here's an overview of the process, why it's important, and what the results could reveal. Types of Microbiome Tests Skin tests: Skin microbiome samples are typically collected using non-invasive methods, such as swabs, tape strips, or skin scrapings. A swab is gently rubbed over the surface of the skin to capture microbial communities from different body areas, depending on the research or diagnostic needs. These samples are then sent to a lab for DNA sequencing to identify and quantify the microbial species present (Grice & Segre, 2011). This approach allows for a deeper understanding of how the skin's microbial balance impacts health conditions like acne, eczema, and skin infections. Saliva tests: Saliva microbiome testing is a non-invasive approach to assess the bacterial communities in the mouth, offering insights into oral and overall health. Through next-generation sequencing, saliva samples can reveal significant shifts in microbial composition, serving as early biomarkers for health conditions. For example, Aas et al. (2005) demonstrated that saliva microbiome testing could reveal distinct bacterial profiles associated with oral diseases, suggesting its importance in both oral and systemic health management. Stool tests: Stool microbiome testing evaluates the gut’s microbial composition by analysing bacteria, viruses, and fungi in stool samples. This non-invasive test helps identify microbial imbalances (dysbiosis) linked to conditions like irritable bowel syndrome, inflammatory bowel disease, and metabolic disorders. By using next-generation sequencing, it reveals the diversity and abundance of gut microbes, which can inform insights into digestive, immune, and even mental health. Zhernakova et al. (2016) highlighted its potential in detecting gut-related health issues and promoting personalised healthcare strategies. Comprehensive clinical tests: In some cases, healthcare providers may recommend a more comprehensive testing that goes beyond the standard saliva and stool samples which include blood tests or even tissue biopsies; particularly when chronic conditions are present. These approaches can hold valuable information regarding systemic infections or localised imbalances in microbiota, which can significantly aid in the management of diseases such as inflammatory bowel disease and even cancer. (Schulfer & Blaser, 2015) Conclusion By testing our microbiome, we dive into important insights of our overall health by identifying imbalances in microbial communities, known as dysbiosis, which are interlinked to various health conditions. Understanding our microbiome composition allows for customised dietary and lifestyle adjustments to restore balance, as specific gut bacteria are essential for digestion, immune function, and mood regulation. (Zhernakova et al., 2016; Young, 2017) Advancements in microbiome research show that testing can aid in managing chronic diseases by guiding personalised treatment and dietary plans based on an individual's microbial profile, improving clinical outcomes and overall well-being (Lloyd-Price et al., 2016; Hu et al., 2021). Regular testing can optimise health, particularly concerning the gut-brain axis and metabolic health. References Aas, J. A., Paster, B. J., Stokes, L. N., Olsen, I., & Dewhirst, F. E. (2005). Defining the normal bacterial flora of the oral cavity. Journal of Clinical Microbiology, 43(11), 5721-5732. https://doi.org/10.1128/JCM.43.11.5721-5732.2005 Lloyd-Price, J., Abu-Ali, G., & Huttenhower, C. (2016). The healthy human microbiome. Genome Medicine, 8(51), 1-11. https://doi.org/10.1186/s13073-016-0307-y Schulfer, A., & Blaser, M. J. (2015). Risks of antibiotic exposures early in life on the developing microbiome. PLoS Pathogens, 11(7), e1004903. https://doi.org/10.1371/journal.ppat.1004903 Zhernakova, A., et al. (2016). Population-based metagenomics analysis reveals markers for gut microbiome composition and diversity. Science, 352(6285), 565-569. https://doi.org/10.1126/science.aad3369