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What is a Microbiome Formulation? What is a Microbiome Formulation? Less is More approach Statistically, Women typically apply around 16 beauty products each day, exposing their skin to about 515 synthetic chemicals daily. (“The Average Woman Uses 16 Beauty Products Every Day. Here Are the Ones Worth the Investment,” 2018) This extensive exposure has led to a rise in skin sensitivity, which now affects 60-70% of women and 50-60% of men worldwide (Sensitive skins wear their own skin microbiota, 2024) - a number that continues to climb. To mitigate these effects, experts advise using fewer, essential ingredients and prioritising simple, balanced formulations over emphasising single "hero" ingredients. This holistic approach fosters skin compatibility, supporting healthier skin with reduced risk of irritation. Traditional vs Microbiome approach The traditional skin care approach highlights a single, "hero" ingredient or a blend of active ingredients to deliver specific benefits, with the spotlight on these components as the main drivers of product efficacy. However, a microbiome-focused approach takes a broader, more holistic perspective. It considers how each ingredient, including non-active additives (functional ingredients), interacts with the skin and its microbiome. This method aims to create formulations that support the skin's natural microbial balance, ensuring that every component contributes positively to overall skin health. Anhydrous Products Anhydrous products, with no water, do not require preservatives since bacteria need moisture to grow. However, they face challenges: incorporating water-soluble actives is difficult, and they are more prone to oxidation and rancidity. Their thick, oil-based consistency may also not suit all skin types, especially oily or sensitive skin. Despite these drawbacks, anhydrous formulations appeal to consumers interested in preservative-free, minimal skincare. Water-based Products Water in skincare products often makes up 80%, requiring emulsifiers to mix with oils and preservatives to prevent bacterial and fungal growth. To avoid contamination, effective preservation is essential. Instead of skipping preservation, reducing water activity can enhance product stability by limiting moisture, which is crucial for safer, less chemically intensive formulations. pH Maintaining a skincare formulation with a slightly acidic pH, ideally below 5, supports the skin's microbiome and barrier function. Alkaline cleansers with pH levels above 7 can disrupt the acid mantle, weakening the barrier and encouraging harmful bacteria. Hülpüsch et al. (2022) found that a higher skin pH (5.7–6.2) in atopic dermatitis patients is linked to increased Staphylococcus aureus colonisation, worsening inflammation and barrier issues. Acidic formulations may help control bacterial overgrowth, reducing flare-ups and promoting skin health in at-risk groups like those with AD. Ingredients Formulations that are high in lipids can strengthen the skin barrier, enhancing hydration and resilience against irritants. Combining gentle surfactants with super fatty agents further reduces potential for irritation and maintains moisture, which is particularly beneficial for sensitive skin types. These principles help prevent disruption of the microbiome, ensuring the skin remains balanced and resilient over time (Van Belkum et al., 2023). The selection of mildly acidic, pH-balanced ingredients close to the skin’s natural acidity (4.7 < pH < 5.7) is crucial, as it helps preserve the skin’s barrier and microbiome. Preservatives To protect the skin microbiome and reduce bacterial growth, it’s essential to minimise preservatives, especially in oil-based products. Anhydrous formulations, which don’t require preservatives, are ideal when possible. For water-based products, alternatives such as antimicrobial peptides (AMPs), and natural humectants like glycerin, sodium lactate, and NMF components (ceramide, urea), can improve microbial stability and skin barrier function (Halla et al., 2018). Airless packaging and sterilisation methods like UHT technology can further enhance product safety by minimising contamination, reducing the need for traditional preservatives. Surfactants Water-in-oil System: Choose emulsifiers with a low hydrophilic-lipophilic balance ratio and natural ingredients like plant oils or sugars. Limit emulsifier concentration to maintain skin barrier integrity. Avoid Polysorbate 80, which can promote pathogen growth, and use alternatives like Polyglyceryl 4 Oleate, which self-emulsify when mixed with water. Oil-in-water System: Use microbiome-friendly emulsifiers derived from natural sources like plant oils and sugars. Examples include Olivem 1000, made from olive oil (Cetearyl Olivate and Sorbitan Olivate), and Emulium Mellifera MB, which combines beeswax and jojoba wax (Polyglyceryl-6 Distearate and Jojoba Esters). These emulsifiers help maintain skin health while supporting the microbiome. It's also important to limit the concentration of emulsifiers to avoid disrupting the skin barrier. Fragrances/Essential Oils Fragrances should generally be avoided in skincare formulations, especially for sensitive skin, as many fragrances can cause irritation. However, certain essential oils may serve as antimicrobial agents in products for non-sensitive skin, offering a natural alternative to synthetic preservatives. For instance, bergamot and lavender oils demonstrate antibacterial and antifungal effects, particularly against Staphylococcus aureus, though they do not affect S. epidermidis (Kim et al., 2022). Additionally, rosemary oil and phenylethyl alcohol show strong antifungal properties, adding preservative benefits without traditional preservatives. Reference Byrd, A. L., Belkaid, Y., & Segre, J. A. (2018). The human skin microbiome. Nature Reviews Microbiology, 16(3), 143-155. Halla, N., Fernandes, I. P., Heleno, S. A., Costa, P., Boucherit-Otmani, Z., Boucherit, K., Rodrigues, A. E., Ferreira, I. C. F. R., & Barreiro, M. F. (2018). Cosmetics Preservation: A Review on Present Strategies. Molecules, 23(7), 1571. https://doi.org/10.3390/molecules23071571 The average woman uses 16 beauty products every day. Here are the ones worth the investment. (2018, December 6). The Telegraph. https://www.telegraph.co.uk/beauty/face/essential-skincare-makeup-products-use-everyday/ Sensitive skins wear their own skin microbiota - BEAUTY HORIZONS 1 2021 WW. (2024, March 27). https://digital.teknoscienze.com/beauty_horizons_1_2021_ww/sensitive_skins_wear_their_own_skin_microbiota Hülpüsch, C., Tremmel, K., Hammel, G., Bhattacharyya, M., De Tomassi, A., Nussbaumer, T., Neumann, A. U., Reiger, M., & Traidl‐Hoffmann, C. (2020). Skin pH–dependent Staphylococcus aureus abundance as predictor for increasing atopic dermatitis severity. Allergy, 75(11), 2888–2898. https://doi.org/10.1111/all.14461 Van Belkum, A., Lisotto, P., Pirovano, W., Mongiat, S., Zorgani, A., Gempeler, M., Bongoni, R., & Klaassens, E. (2023). Being friendly to the skin microbiome: Experimental assessment. Frontiers in Microbiomes, 1. https://doi.org/10.3389/frmbi.2022.1077151 Nielsen, H. L., et al. (2016). Influence of emulsifiers on microbial stability in cosmetic formulations. International Journal of Cosmetic Science, 38(4), 357-366. https://doi.org/10.1111/ics.12301 Kim, J., et al. (2022). Antimicrobial effects of essential oils on skin microbiota. Journal of Cosmetic Science, 73(5), 452-460.

< Back Sales Associate (UK Based) United Kingdom Job Type Full-time Workspace Hybrid About the Role As a Sales Associate at Sequential, you will be essential in building relationships and expanding our reach within the microbiome industry. Your role will involve supporting business development efforts, assisting in sales strategy execution, and helping grow market share for Sequential’s innovative products and services. You will work closely with the Commercial Director and team to meet sales goals, develop client relationships, and contribute to Sequential’s mission of advancing microbiome science. This is a fantastic opportunity for an ambitious sales professional to gain hands-on experience and make a tangible impact in a rapidly growing biotech field. Essential Duties and Responsibilities: Sales Support and Development: Assist in the identification and acquisition of new customer accounts, supporting the growth of Sequential’s client base. Client Relationship Building: Cultivate relationships with existing and prospective clients, focusing on understanding their needs and promoting Sequential’s unique offerings. Market Research: Conduct ongoing research to stay informed on industry trends and competitor activity, providing valuable insights for sales strategies. CRM and Data Management: Maintain accurate records in our CRM (HubSpot), tracking interactions, progress, and key performance indicators. Sales Target Achievement: Work towards defined sales goals, contributing to Sequential’s revenue targets and growth ambitions. Collaborative Efforts: Work closely with marketing, product, and operations teams to align efforts and ensure a seamless customer experience. Product Knowledge Development: Stay up-to-date on Sequential’s product offerings and the latest microbiome research to effectively communicate value to clients. Qualifications and Experience: 1-2 years of sales experience, ideally within the biotechnology, cosmetics, or life sciences fields. Familiarity with CRM systems, preferably HubSpot, and the ability to manage data with accuracy. Strong written and verbal communication skills with a passion for client interaction and customer service. Tech-savvy and eager to leverage technology to enhance sales effectiveness. Bachelor's degree in Life Sciences, Business, or a related field preferred; demonstrated interest in microbiome or biotechnology is a plus. Motivated self-starter with a goal-oriented mindset and excellent organizational skills. Ability to work collaboratively and adapt in a dynamic, fast-paced environment. What You Get from Us: A base salary with a commission structure that rewards your hard work and success. Access to our Equity Incentive Plan, allowing you to grow alongside Sequential as we expand. The chance to work with industry experts in one of the fastest-growing sectors within biotechnology. Be part of a company at the forefront of microbiome research, making an impact in the biotechnology and healthcare fields. About the Company Sequential is a global leader in the skin microbiome field, a team of PhD experts in testing products and their effect on the human microbiome (skin, scalp, oral, vulva). An Innovate UK- and Enterprise Singapore-backed company, with labs in London, New York City and Singapore. Awarded the title "Most Significant" Testing Solution in the Industry - (C&T, 2022). To date, Sequential has amassed over 25,000 human skin microbiome samples and corresponding formulations tested in vivo on the skin. With this vast genomic dataset, Sequential are figuring out optimal formulations that could potentially alleviate skin conditions, for example – acne, atopic dermatitis and rosacea. They have been supported by Innovate UK, Enterprise SG, A*STAR (Genome Institute of Singapore). They have raised $4.3MM USD to date from SOSV, Metaplanet, Scrum Ventures, Corundum Systems Biology and are a resident company of JLABS Innovation (in NYC). Apply Now

Understand Sequential's terms and conditions for using our website. Terms Of Use Last updated: 13 March 2024 Please read these terms of use ("Terms", "Terms of Use") carefully before using the www.sequential.bio website (the "Service") operated by Sequential Skin Ltd ("us", "we", or "our"). Your access to and use of the Service is conditioned on your acceptance of and compliance with these Terms. These Terms apply to all visitors, users, and others who access or use the Service. By accessing or using the Service, you agree to be bound by these Terms. If you disagree with any part of the terms, then you may not access the Service. Accounts When you create an account with us, you must provide us with information that is accurate, complete, and current at all times. Failure to do so constitutes a breach of the Terms, which may result in immediate termination of your account on our Service. You are responsible for safeguarding the password that you use to access the Service and for any activities or actions under your password, whether your password is with our Service or a third-party service. You agree not to disclose your password to any third party. You must notify us immediately upon becoming aware of any breach of security or unauthorized use of your account. Intellectual Property The Service and its original content, features, and functionality are and will remain the exclusive property of Sequential Skin Ltd and its licensors. The Service is protected by copyright, trademark, and other laws of both the UK and foreign countries. Our trademarks and trade dress may not be used in connection with any product or service without the prior written consent of Sequential Skin Ltd. Links To Other Web Sites Our Service may contain links to third-party web sites or services that are not owned or controlled by Sequential Skin Ltd. Sequential Skin Ltd has no control over, and assumes no responsibility for, the content, privacy policies, or practices of any third-party web sites or services. You further acknowledge and agree that Sequential Skin Ltd shall not be responsible or liable, directly or indirectly, for any damage or loss caused or alleged to be caused by or in connection with use of or reliance on any such content, goods, or services available on or through any such web sites or services. We strongly advise you to read the terms and conditions and privacy policies of any third-party web sites or services that you visit. Termination We may terminate or suspend your account immediately, without prior notice or liability, for any reason whatsoever, including without limitation if you breach the Terms. Upon termination, your right to use the Service will immediately cease. If you wish to terminate your account, you may simply discontinue using the Service. Governing Law These Terms shall be governed and construed in accordance with the laws of England and Wales, without regard to its conflict of law provisions. Our failure to enforce any right or provision of these Terms will not be considered a waiver of those rights. If any provision of these Terms is held to be invalid or unenforceable by a court, the remaining provisions of these Terms will remain in effect. These Terms constitute the entire agreement between us regarding our Service, and supersede and replace any prior agreements we might have between us regarding the Service. Changes We reserve the right, at our sole discretion, to modify or replace these Terms at any time. If a revision is material, we will try to provide at least 30 days' notice prior to any new terms taking effect. What constitutes a material change will be determined at our sole discretion. By continuing to access or use our Service after those revisions become effective, you agree to be bound by the revised terms. If you do not agree to the new terms, please stop using the Service. Contact Us If you have any questions about these Terms, please contact us: - By email: team@sequential.bio - By visiting this page on our website: www.sequential.bio

How do I Rebuild my Skin Microbiome? How do I Rebuild my Skin Microbiome? Our skin, as we know to be the body's largest organ, hosts a plethora of microorganisms. An elaborate ecosystem of bacteria, fungi, and viruses that live on the skin and maintain its optimal health, making up the skin microbiome (Smythe & Wilkinson, 2023). These microorganisms serve as a protective barrier against harmful pathogens, aid in immune system regulation, and play a vital role in various skin functions. However, factors such as harsh skincare products, poor diet, and environmental stressors can disrupt the balance, leading to acne, eczema, and other skin disorders (Wallen-Russell, 2019). Restoring the skin microbiome involves a complex and multifaceted approach that includes lifestyle changes, targeted skincare practices, and sometimes dietary adjustments. Understanding the Skin Microbiome The microbiome varies depending on the region of the skin, with different areas hosting distinct microbial communities. Fournière et al. (2020) examine how Staphylococcus epidermidis and Cutibacterium acnes, key skin microbiota components, vary across different skin environments. For instance, Staphylococcus species are mainly in moist areas like axillary and popliteal creases, while Cutibacterium species are found in sebaceous areas such as the face and back. An ideal and healthy skin microbiome is diverse, and resilient, capable of adapting to changes and defending against adversaries. Steps to Restore the Skin Microbiome 1. Use Less & Gentle Skincare Products: Using lesser products would simplify one’s skincare routine, making it easier to stay consistent and therefore allow the skin to adjust and benefit from the active ingredients. Hwang et al. (2021) further suggests that in order to restore the skin microbiome, one should actively choose products with essential ingredients which are natural, soothing and non-disruptive to the skin's natural barrier. 2. Incorporate Probiotics, Prebiotics and Postbiotics: Just as the gut microbiome benefits from probiotics, so does the skin. Probiotic skincare adds beneficial bacteria to restore skin balance, while prebiotics nourish existing good bacteria to boost their growth. Common probiotics include Lactobacillus and Bifidobacteria, found in drinks, powders, tablets, and fermented dairy products (Gowda, et al. 2024). 3. Protect Your Skin from Environmental Stressors: Limit exposure to environmental pollutants and the sun, which can damage the skin barrier and disrupt the microbiome (Wang et al., 2021). Using protective measures like sunscreen, wearing protective clothing, and cleansing your skin regularly can help maintain the integrity of the skin barrier and support a balanced microbiome. 4. Moisturise Regularly: Keeping the skin well-hydrated is crucial for maintaining a healthy microbiome. Dry skin can compromise the skin barrier, making it easier for harmful bacteria to invade. Use a moisturiser that contains ingredients like ceramides, which help to strengthen the skin barrier, and hyaluronic acid to retain moisture. (Spada & Greive, 2018) 5. Pay Attention to Diet: A diet rich in fruits, vegetables, and whole grains supports skin microbiome health with essential vitamins, minerals, and antioxidants. Omega-3 fatty acids, found in fish and flaxseeds, are particularly beneficial as they have anti-inflammatory properties that can help maintain a balanced skin microbiome. (Costantini, Molinari & Merendino, 2017) Conclusion Restoring the skin microbiome is an ongoing process that demands consistent effort and dedication. By understanding the skin microbiome and incorporating strategies to support its health, you can significantly enhance your skin's ability to protect against environmental stressors and lower the risk of skin disorders. References Smythe, P., & Wilkinson, H. N. (2023). The skin microbiome: Current landscape and future opportunities. International Journal of Molecular Sciences, 24(4), 3950. https://doi.org/10.3390/ijms24043950 Wallen-Russell, C. (2019). The impact of skin care products on skin chemistry and microbiome dynamics. BMC Biology, 17(1), 47. https://doi.org/10.1186/s12915-019-0660-6 Fournière, M., Latire, T., Souak, D., Feuilloley, M. G. J., & Bedoux, G. (2020). Staphylococcus epidermidis and Cutibacterium acnes: Two major sentinels of skin microbiota and the influence of cosmetics. Microorganisms, 8(11), 1752. https://doi.org/10.3390/microorganisms8111752 Hwang, B. K., Lee, S., Myoung, J., Hwang, S. J., Lim, J. M., Jeong, E. T., Park, S. G., & Youn, S. H. (2021). Effect of the skincare product on facial skin microbial structure and biophysical parameters: A pilot study. MicrobiologyOpen, 10(5), e1236. https://doi.org/10.1002/mbo3.1236 Gowda, V., Sarkar, R., Verma, D., & Das, A. (2024). Probiotics in Dermatology: An Evidence-based Approach. Indian dermatology online journal, 15(4), 571–583. https://doi.org/10.4103/idoj.idoj_614_23 Wang, L., Xu, Y. N., Chu, C. C., Jing, Z., Chen, Y., Zhang, J., Pu, M., Mi, T., Du, Y., Liang, Z., Doraiswamy, C., Zeng, T., Wu, J., & Chen, L. (2021). Facial Skin Microbiota-Mediated Host Response to Pollution Stress Revealed by Microbiome Networks of Individual. mSystems, 6(4), e0031921. https://doi.org/10.1128/mSystems.00319-21 Spada, F., Barnes, T. M., & Greive, K. A. (2018). Skin hydration is significantly increased by a cream formulated to mimic the skin's own natural moisturizing systems. Clinical, cosmetic and investigational dermatology, 11, 491–497. https://doi.org/10.2147/CCID.S177697 Costantini, L., Molinari, R., Farinon, B., & Merendino, N. (2017). Impact of Omega-3 Fatty Acids on the Gut Microbiota. International journal of molecular sciences, 18(12), 2645. https://doi.org/10.3390/ijms18122645

How do you Formulate Skincare Products? How do you Formulate Skincare Products? Formulating skincare products is a process that combines different ingredients to provide targeted skin benefits. The type of product being used, the particular skin concern and the formulation's stability and effectiveness are some of the variables that can affect the formulation process. Key Components of Skincare Formulation: Choosing the right ingredients when formulating skincare products is important, as it determines the product's effectiveness. Moreover, the chemical composition of the ingredients and their concentrations in a formulation is as important as establishing its overall effect on our health. Most skincare products typically include 15 to 20 ingredients in their formulation (Goyal & Jerold, 2023). Water Ultra-pure distilled water serves as the foundation for nearly every cosmetic formulation, including creams, lotions, makeup, deodorants, shampoos, and conditioners. It functions primarily as a solvent, helping to emulsify ingredients and ensure a smooth and consistent texture in the final product (Goyal & Jerold, 2023). Emulsifiers Promotes the formation of a homogenous and consistent texture in the formulation by encouraging the suspension of water and oil (Goyal & Jerold, 2023). Humectants Humectants are crucial for the skin's hydration balance, moisture retention, and desiccation prevention. Multiple hydroxyl, carboxyl, or other polar groups found in humectants have the ability to create hydrogen bonds with water molecules, which allows them to absorb and hold onto moisture (Du et al., 2024). Thickeners Long carbon chains and polar groups like hydroxyl, carboxyl, and amide groups make up most of the thickeners. Within solutions, these long-chain structures increase the viscosity of the solution by increasing its resistance to flow (Du et al., 2024). Preservatives Preservatives are added to prevent the growth of pathogenic and non-pathogenic microorganisms that could degrade the product or negatively impact the consumer's health De et al., 2017). Active Ingredients Ingredients that offer therapeutic advantages and improve the efficacy of skincare products, such as hyaluronic acid, are used as active components in formulations (Morganti et al., 2023). Therefore, selecting the appropriate active ingredient is crucial to creating a high-quality skin care product (Calixto & Maia, 2017). Stability testing and sensory analysis The formulations afterwards need to be tested for their stability at different temperatures and for sensory evaluation such as spreadability, cohesiveness, consistency, firmness and viscosity (Calixto & Maia, 2017). Targeting the microbiome with Skincare products Our skin is home to millions of microbial species called the Skin microbiome. A balance of all these microbes is crucial to obtain healthy skin. Imbalances cause several skin problems such as acne, rosacea…etc. So when formulating skincare products it is important to investigate each ingredient's impact on the skin microbiome. Conclusion It is of great importance to carefully consider the ingredients that are put into skincare formulations whilst considering the skin microbiome. Developing safe and efficient products is frequently the main goal, there is also a growing tendency towards customisation based on the demands of each individual's skin. This trend towards customised skincare highlights the value of customised solutions in the beauty industry and represents a deeper awareness of the various needs of various skin types and conditions. References Calixto LS, Maia Campos PMBG. Physical-Mechanical characterization of cosmetic formulations and correlation between instrumental measurements and sensorial properties. Int J Cosmet Sci. 2017 Oct;39(5):527-534. doi: 10.1111/ics.12406. Epub 2017 Jul 24. PMID: 28555924. De Groot, A. C., & White, I. R. (2001). Cosmetics and Skin Care Products. Textbook of Contact Dermatitis, 661–685.doi:10.1007/978-3-662-10302-9_32 Du XN, He Y, Chen YW, Liu Q, Sun L, Sun HM, Wu XF, Lu Y. Decoding Cosmetic Complexities: A Comprehensive Guide to Matrix Composition and Pretreatment Technology. Molecules. 2024 Jan 15;29(2):411. doi: 10.3390/molecules29020411. PMID: 38257324; PMCID: PMC10818968. Goyal N, Jerold F. Biocosmetics: technological advances and future outlook. Environ Sci Pollut Res Int. 2023 Feb;30(10):25148-25169. doi: 10.1007/s11356-021-17567-3. Epub 2021 Nov 25. PMID: 34825334; PMCID: PMC8616574. Morganti, P.; Lohani, A.; Gagliardini, A.; Morganti, G.; Coltelli, M.-B. Active Ingredients and Carriers in Nutritional Eco-Cosmetics. Compounds 2023, 3, 122-141. https://doi.org/10.3390/compounds3010011

Sequential is proud to announce that is has been dubbed the most significant testing method in the industry for microbiome testing. Allē Award: Sequential Wins Prestigious “Most Significant” Testing Method After being listed as a finalist as best ‘Claims testing methods & tools’ alongside Evonik, and XCellR8 - Sequential brings home top prize in C&T’s Allē Awards, 2022. The personal care and cosmetics industry in the US is valued at $190B. In the context of the skin microbiome, the industry is still a relatively nascent field. However, its projected growth is significant, estimated to grow at a compounded annual growth of 29.2% between 2022-2028. Owed to the impact of products on the skin microbiome, consumer awareness, and understanding that our microbiome is intimately linked to our health. Sequential is the B2B microbiome testing arm for Sequential Skin Ltd, which has developed the world’s first end-to-end platform for companies to evaluate their products on the microbiome in vivo. They specialize in skin, scalp and intimate area microbiome. "Of all the 'microbiome friendly' methodologies working to address both consumer and industry concerns about the impact of cosmetics on the microbiome, this is, in my opinion, the most quantitative and promising of the lot." – C&T's Allē Award judges, 2022. The company has developed a proprietary non-invasive method for collecting skin samples to analyze the skin microbiome using next-generation sequencing (NGS), with species and strain identification, in longitudinal (and clinical) studies. Services include data interpretation and the results are reported in a comprehensive, yet understandable format, ready for formulators and research scientists to incorporate into their product development pipeline. “The Allē Award gives us strong confidence in the testing platform and capabilities we’ve built in Sequential. Being the first to develop an in vivo microbiome test for the industry, we’re proud to see our hard work paying off” — Petronille Houdart, DPharm, skincare director at Sequential. About Sequential Sequential is part of the Sequential Skin group – with a US lab in New York City, alongside a lab in London and a lab in Singapore. Sequential team has over 20 years of combined expertise in genetics, epigenetics, and microbiome research. Sequential has validated its AI-driven testing platform with over 30 companies, analyzing over 12,000 skin microbiome samples. They specialise in skin, scalp and vulva/vaginal microbiome samples. They are supported by Enterprise SG, A*STAR, Genome Institute of Singapore, IndieBio New York, SOSV, Metaplanet Holdings, Scrum Ventures, Genedant VC, Ben Holmes (ex. General Partner at Index Ventures), Innovate UK, and are a resident company of Johnson & Johnson Innovation – JLABS.

Rosacea is a chronic skin condition affecting an estimated 16 million Americans and millions more worldwide, primarily adults over 30 (National Rosacea Society). TARGETED SKIN PANEL Rosacea Targeted Panel 23 Smart Probes ™ Rosacea Panel Validated targets: Demodex mites, Bacillus oleronius, Corynebacterium kroppenstedtii Absolute Quantification End-to-end Service Qet a Quote Unlock the Secrets of Rosacea with Sequential's Cutting-Edge Microbiome Testing Why Microbiome Testing for Rosacea? Rosacea is a chronic skin condition affecting an estimated 16 million Americans and millions more worldwide, primarily adults over 30 (National Rosacea Society). Characterized by redness, visible blood vessels, and inflammation, rosacea can significantly impact an individual’s quality of life. Research suggests that the skin microbiome plays a crucial role in the development and severity of rosacea. Specifically, imbalances in microbial populations, such as an overgrowth of Demodex mites or harmful bacteria, can exacerbate symptoms. In vivo testing of skincare products for rosacea is essential to ensure that formulations are compatible with the unique microbiome of rosacea-prone skin. Such testing allows researchers to observe real-world effects on microbial balance and inflammation, ensuring that products do not unintentionally worsen symptoms. Without this careful testing, products may disrupt the microbiome further, leading to increased redness, irritation, or flare-ups. Sequential is leading the way in microbiome testing for the industry. With a specialized focus on skin conditions, we have developed an innovative solution tailored to help you understand the root causes of rosacea at the microbial level. Our proprietary sequencing panel is designed specifically to offer in-depth analysis of rosacea-associated microbiota, giving you the tools to transform skincare solutions. Customized Analysis Tailored insights specific to rosacea-prone skin, allowing your R&D team to develop more personalized and effective products. Actionable Data Leverage detailed reports to guide formulation decisions and validate product efficacy with precision. Product Innovation New rosacea treatments or enhancing existing lines, our data helps you create targeted solutions with proven results. Precision Sequencing Target specific microbial communities associated with rosacea development and severity. Smart Probe s ™ Our dedicated team of scientists has developed a revolutionary method for evaluating microbes through our Smart Probes™, a proprietary panel that includes 23 key microbes specifically identified as having the most significant impact on skin health. To further understand the complex relationship between rosacea and the skin microbiome, we utilize qPCR technology. This advanced method allows us to accurately quantify microbial populations on the skin, offering deeper insights into how rosacea affects the balance of beneficial and harmful microbes. By closely monitoring how skincare products interact with these microbes, companies can develop solutions that not only target rosacea symptoms but also help restore a healthier, balanced skin microbiome, providing long-term relief for sufferers. Rosacea Panel Targets Demodex mites (Demodex folliculorum and Demodex brevis) These tiny mites are normally present on the skin but are often found in higher numbers on the skin of individuals with rosacea. Their presence is thought to trigger inflammatory responses. Forton FMN. The Pathogenic Role of Demodex Mites in Rosacea: A Potential Therapeutic Target Already in Erythematotelangiectatic Rosacea? Dermatol Ther (Heidelb). 2020 Dec;10(6):1229-1253. doi: 10.1007/s13555-020-00458-9. Epub 2020 Oct 23. PMID: 33095403; PMCID: PMC7649190. Kim HS. Microbiota in Rosacea. Am J Clin Dermatol. 2020 Sep;21(Suppl 1):25-35. doi: 10.1007/s40257-020-00546-8. PMID: 32914214; PMCID: PMC7584533. Bacillus oleronius This bacterium has been linked to rosacea, particularly in relation to the inflammatory response associated with the condition. It is often found in higher quantities on the skin of rosacea patients. Corynebacterium kroppenstedtii This bacterium appears to be significantly overrepresented in papulopustular and mixed rosacea compared to healthy skin, with its presence correlating with disease severity and lesion spread, suggesting a potential opportunistic or contributory role in rosacea pathophysiology. Chien AL, Kang S, et al. Characterization and Analysis of the Skin Microbiota in Rosacea. J Am Acad Dermatol. 2020; PMID: 31502207. Industry Leader in Absolute Quantification Sequential’s Smart Probe ™ qPCR approach to microbiome analysis can resolve species and subspecies, giving statistically significant findings between skin conditions on in vivo human samples, compared to conventional sequencing approaches. Relevant Research How Does the Skin Microbiome Influence Rosacea? Unveiling the Microbial Puzzle Read More Articles

Elevate your brand with skin microbiome science. Gain a competitive edge with certified, innovative personal care products rooted in robust research. in vivo Sampling is the Future of Microbiome Testing We lead in clinical microbiome research and testing offering a comprehensive end-to-end platform designed to bring science-backed solutions to the personal care and pharma industry. We offer an expansive platform to conduct research on personal care products through microbiome testing, biophysical assessments, and offer full recruitment services for studies. We are keen to publish our findings with our partners to increase the literature within this space. At present our database of over 20,000 human microbiome samples is one of the most sophisticated within the industry and is growing rapidly. Skin Test your skincare products, ranging from facial cleansers, serums, moisturisers, and SPFs. Scalp Test your scalp care products ranging from scalp serums, oils, shampoos, and conditioners. Vaginal Test your intimate care products, ranging from washes, to hygiene sprays, and balms. Oral Test your oral care products ranging from mouth washes and toothpastes. Full End-To-End Testing Platform in vivo Study Design Collaborating closely with our clients to develop customised study protocols, ensuring that objectives are met with precision. Validated Collection Our non-invasive collection system consistently collects material for downstream analysis and allows for remote testing. Analysis & Reporting All analysis is carried out in-house allowing for a faster turn-around time on your study with exclusive access to our team of scientists and formulation experts. White Paper Publication Co-author a white paper with us based on the findings of your study to shorten the knowledge gap within the industry to demonstrate active's impact. Candidate Recruitment Carefully select human participants (location, age, etc.) to ensure the relevance and accuracy of the study. Microbiome Detection We offer four types of sequencing techniques including qPCR with our proprietary Smart Probes™, 16S, ITS and Shotgun Metagenomics. Claims Certification Providing comprehensive reports and certifications (Maintains the Microbiome seal) based on the study results which can be used in communication efforts. Co-Presentation We offer support by co-presenting at conferences where you want to demonstrate the impact of your study findings and explain the science behind the research. Start Your Clinical Study Today! State of the Art Sequencing Using next-generation sequencing of the collection of micro-organisms found on the body, during product usage, Sequential investigates the microbial diversity, and particular micro-organisms we know are important and play a role in a healthy microbiome. With your pick from one of four sequencing reports, Sequential makes it easier to understand what depth of analysis is required for your particular needs. qPCR Microbiome (Bacteria+Fungi) Quantitative PCR (qPCR), also known as Real-Time PCR, is a method that Sequential uses in-house to measure the absolute number of copies of a microbial DNA defined by Sequential’s proprietary Smart Probes™. Using this method, we quantify the amount bacteria or fungi, allowing us to report level of species and strains within a given sample. The entire process is done at Sequential to ensure quality control, and extra control on your samples and privacy. Up Next-Generation Sequencing of ITS (total fungal population) ITS rRNA gene sequencing, or ITS amplicon sequencing is performed at Sequential to determine the relative abundance of taxa in a fungal community, and to compare between groups of interest. We know that fungi play a critical role in the microbiome of a healthy human, and it’s important to consider this application, especially for the scalp microbiome, and vaginal or vulva microbiome. We routinely offer two types of ITS rRNA primer regions: ITS1 & ITS2 Up Next-Generation Sequencing of 16S 16S rRNA gene sequencing, or 16S amplicon sequencing, is performed at Sequential to determine the relative abundance of taxa in a bacterial community, and to compare between groups of interest. This level of analysis can help to address changes in the overall bacterial profile over time or between treatment groups. We routinely offer two types of 16s rRNA primer regions: 16S V4 & 16S V1-V3 Up Shotgun Metagenomic Sequencing Shotgun metagenome sequencing is performed at Sequential for taxonomic profiling (diversity and abundance), as well as functional analysis across different organisms in a more exploratory approach. This complex technique allows for parallel sequencing of DNA from all organisms (bacterial, fungal, viral and human) within the community, with good coverage for species-level detection. The data generation allows for more advanced reporting and genome assemblies. Up FAQ What is Sequential's testing platform? Sequential has developed the gold standard test for microbiome-friendly products, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. We offer a complete end-to-end solution to support microbiome-friendly claims. From consultancy and study design to our proprietary microbiome testing kits. We analyse, interpret and report our findings to meet your needs. Why is it necessary to test the microbiome in vivo? At present, there are no regulations for microbiome-related formulas that brands and formulators can follow, however, it has been universally acknowledged that the in vivo method of conducting clinical studies is becoming critical and paramount to getting marketing claims through. When regulations are introduced, which may be imminent, the in vitro system will find itself lacking, resulting in limited claims and certifications that do not hold their value. This is why, we at Sequential strive to offer an in vivo approach, knowing full well that we want our client's claims to be significantly backed by scientific and quantifiable data. What type of sequencing technology does Sequential use for analysis? We offer four types of sequencing techniques including qPCR with our Smart Probes™, 16S, ITS and Shotgun Metagenomics. Using next-generation sequencing of the collection of microorganisms found on the body, during product usage, Sequential investigates the microbial diversity, and particular microorganisms we know are important and play a role in a healthy microbiome. Does Sequential offer claims certification for tested products? We provide our clients with a certification to claim “Maintains the Microbiome” subject to in vivo testing results which can be used in communication efforts. Once your product is tested with our qPCR Smart Probes™ and has shown favourable results in supporting the microbiome, we can certify your product with our Maintains the Microbiome certification seal. We have ensured that our seal and certification are backed by quantifiable data and scientifically significant markers. The aim is to ensure our clients feel confident in making their claims and can communicate the true benefit of their microbiome formulations.

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What are the Best Microbiome Tests? What are the Best Microbiome Tests? As interest in the human microbiome grows, various microbiome tests have emerged, each offering unique insights into our health. Understanding which tests are best suited for individual needs can help inform decisions regarding health management and lifestyle changes. Skin Microbiome Testing Skin microbiome testing involves collecting samples from the skin's surface using methods like swabs, tape strips, or skin scrapings to analyse the diversity and composition of microorganisms residing on the skin. The samples are then processed through DNA sequencing techniques to identify microbial species and their relative abundance. This testing provides insights into how the skin's microbial ecosystem impacts health conditions such as acne and eczema. Skin microbiome research is increasingly being used to develop personalised skincare products and therapeutic approaches. (Byrd et al., 2018) Stool Microbiome Testing Stool testing remains one of the most common and accessible methods for analysing the gut microbiome. These tests evaluate the diversity and abundance of microbial species, helping to identify imbalances linked to conditions such as irritable bowel syndrome (IBS), inflammatory bowel disease (IBD), and obesity. A study by Zhernakova et al. (2016) emphasises the utility of stool microbiome tests in detecting dysbiosis and understanding its relationship to health outcomes. Saliva Microbiome Testing Saliva microbiome testing is another non-invasive method, focusing on the oral microbiota. This test can provide insights into oral health, periodontal disease, and even systemic health conditions linked to the oral microbiome. A study by Aas et al. (2005) illustrates that the oral microbiome reflects changes in health status, making saliva testing an essential tool for early disease detection. Blood Microbiome Testing While less common, blood microbiome testing is gaining traction, especially for identifying systemic infections or chronic conditions. This testing method analyses microbial DNA present in the bloodstream, which can reveal insights into conditions like sepsis and cardiovascular diseases. A paper by Cheng et al. (2023) reviews the emerging concept of the blood microbiome, highlighting its potential role in human health and disease, while addressing existing controversies and the need for further research to understand its clinical implications. Tissue Biopsy Microbiome Testing Tissue biopsies are increasingly utilised for studying the microbiome within specific organs, particularly the gastrointestinal tract. This method allows for direct analysis of microbial communities in tissue samples, providing insights into diseases such as colorectal cancer and IBD. Recent advancements in next-generation sequencing have enhanced the ability to identify and characterise microbial populations in biopsies, as noted by Schulfer and Blaser (2015). Conclusion Choosing the right microbiome test depends on individual health concerns and goals. Stool and saliva tests are excellent for general gut and oral health insights, while blood and tissue tests offer deeper analysis for chronic diseases. As research continues to evolve, these tests will likely become even more integral to personalised healthcare and preventative strategies. References Aas, J. A., Paster, B. J., Stokes, L. N., Olsen, I., & Dewhirst, F. E. (2005). Defining the normal bacterial flora of the oral cavity. Journal of Clinical Microbiology, 43(11), 5721-5732. https://doi.org/10.1128/JCM.43.11.5721-5732.2005 Byrd, A. L., Belkaid, Y., & Segre, J. A. (2018). The human skin microbiome. Nature Reviews Microbiology, 16(3), 143-155. https://doi.org/10.1038/nrmicro.2017.157 Cheng, H. S., Tan, S. P., Wong, D. M. K., Koo, W. L. Y., Wong, S. H., & Tan, N. S. (2023). The blood microbiome and health: Current evidence, controversies, and challenges. International Journal of Molecular Sciences, 24(6), 5633. https://doi.org/10.3390/ijms24065633 Schulfer, A., & Blaser, M. J. (2015). Risks of antibiotic exposures early in life on the developing microbiome. PLoS Pathogens, 11(7), e1004903. https://doi.org/10.1371/journal.ppat.1004903 Zhernakova, A., Kurilshikov, A., Bonder, M. J., Tigchelaar, E. F., Schirmer, M., Vatanen, T., ... & Wijmenga, C. (2016). Population-based metagenomics analysis reveals markers for gut microbiome composition and diversity. Science, 352(6285), 565-569. https://doi.org/10.1126/science.aad3369

What Is a Skin Microbiome Kit? What Is a Skin Microbiome Kit? A skin microbiome test kit is a diagnostic tool designed to analyse the microorganisms living on the surface of the skin. These kits assess the diversity and abundance of bacteria, fungi, and other microbes, providing insights into the skin’s microbial ecosystem. The information gathered can help individuals and healthcare providers understand microbial imbalances that might contribute to dermatological conditions or identify strategies to improve skin health. Components of a Skin Microbiome Kit Sampling Materials Swabs: Sterile swabs are used to collect skin samples from specific areas, such as the face, hands, or scalp. Adhesive Strips: In some kits, tape-stripping methods collect deeper microbial samples by gently removing skin cells along with surface microbes. Sample Preservation The collected samples are placed into a vial or test tube containing a stabilising agent to preserve the DNA of microbes until the sample reaches the laboratory. Instructions for Use Clear instructions are included to guide users on how to collect samples correctly to avoid contamination and ensure accurate results. Once the sample arrives at the lab, it undergoes next-generation sequencing (NGS) or polymerase chain reaction (PCR) analysis. These techniques identify microbial DNA and quantify the presence of various microorganisms. (Nafea et al., 2024) Results are typically shared in reports, offering detailed insights into the composition of the user’s skin microbiome and recommendations for skincare or lifestyle adjustments. Applications and Benefits -Personalised skincare: Tailored treatments based on an individual’s skin microbial profile. By identifying specific bacteria or fungi, users can specifically customise their skincare routines, using products designed to promote a healthy microbiome or address specific concerns such as acne or dryness. Companies may also use the microbiome data collected to formulate more effective skincare products in the future. -Identifying dysbiosis: Kits can detect microbial imbalances (dysbiosis), which are associated with conditions such as eczema, acne, and psoriasis. (Borrego-Ruiz & Borrego, 2024) This allows dermatologists to recommend targeted therapies, such as probiotics or prebiotics, to restore balance and improve skin health. (D’Auria et al., 2021) They are also helpful for tracking the effectiveness of treatments over time. -Monitoring the impact of lifestyle changes: The skin microbiome is influenced by environmental factors like humidity, pollution, and UV exposure, as well as lifestyle choices such as diet and stress management. Testing allows users to monitor how these factors affect their microbial health and make adjustments accordingly. (Byrd et al., 2018) -Research purposes: Helping dermatologists and researchers understand the dynamics of skin health better and test the effectiveness of new treatments. This data can drive the development of these new therapies, including microbiome-based interventions, and help identify potential biomarkers for skin disorders. (D’Auria et al., 2021) Scientific Significance The skin microbiome plays a crucial role in maintaining the skin's barrier function and immune responses. Imbalances in these microbial communities are often associated with dermatological conditions, and research continues to explore how restoring balance through topical or oral interventions could improve skin health. Accurate sampling and analysis, however, remain critical challenges for researchers, requiring reproducible methods for reliable results. (D’Auria et al., 2021) Conclusion A skin microbiome kit offers a gateway into understanding the complex ecosystem on our skin. While still emerging as a mainstream tool, these kits are becoming popular in both consumer skincare and clinical research. As more studies focus on the interplay between the skin microbiome and external factors such as diet and environmental exposures, personalised skincare routines may soon become the norm. References Byrd, A. L., Belkaid, Y., & Segre, J. A. (2018). The human skin microbiome. Nature Reviews Microbiology, 16(3), 143-155. https://doi.org/10.1038/nrmicro.2017.157 Borrego-Ruiz, A., & Borrego, J. J. (2024). Microbial Dysbiosis in the Skin Microbiome and Its Psychological Consequences. Microorganisms, 12(9), 1908. https://doi.org/10.3390/microorganisms12091908 D'Auria, E., Acunzo, M., et al. (2021). A journey on the skin microbiome: Pitfalls and opportunities. International Journal of Molecular Sciences, 22(18), 9846. https://doi.org/10.3390/ijms22189846 Nafea, A. M., Wang, Y., Wang, D., Salama, A. M., Aziz, M. A., Xu, S., & Tong, Y. (2024). Application of next-generation sequencing to identify different pathogens. Frontiers in Microbiology, 14. https://doi.org/10.3389/fmicb.2023.1329330

Acne affects nearly 85% of people between the ages of 12 and 24, with millions worldwide continuing to struggle with breakouts into adulthood (American Academy of Dermatology). TARGETED SKIN PANEL Acne Targeted Panel 23 Smart Probes ™ Acne Panel Validated targets: Cutibacterium acnes acnes (subtype I) Cutibacterium acnes defendens (subtype II), Cutibacterium acnes elongatum (subtype III) Absolute Quantification End-to-end Service Qet a Quote Unlock the Secrets of Acne with Sequential's Cutting-Edge Microbiome Testing Why Microbiome Testing for Acne? Acne affects nearly 85% of people between the ages of 12 and 24, with millions worldwide continuing to struggle with breakouts into adulthood (American Academy of Dermatology). While many individuals turn to over-the-counter products in hopes of finding relief, the reality is that many of these formulations can exacerbate the problem. Without proper testing, products may contain ingredients that disrupt the delicate balance of the skin, leading to irritation, clogged pores, and even more severe acne. Acne isn’t just skin-deep — it’s driven by a delicate balance of microorganisms on the skin. As skincare companies race to meet demand, a lack of microbiome-focused testing means many products fail to address the root microbial causes of acne. Sequential is leading the way in microbiome testing for the industry. With a specialized focus on skin conditions, we have developed an innovative solution tailored to help you understand the root causes of acne at the microbial level. Our proprietary sequencing panel is designed specifically to offer in-depth analysis of acne-associated microbiota, giving you the tools to transform skincare solutions. Customized Analysis Tailored insights specific to acne-prone skin, allowing your R&D team to develop more personalized and effective products. Actionable Data Leverage detailed reports to guide formulation decisions and validate product efficacy with precision. Product Innovation New acne treatments or enhancing existing lines, our data helps you create targeted solutions with proven results. Precision Sequencing Target specific microbial communities associated with acne development and severity. Smart Probe s ™ Our dedicated team of scientists has developed a groundbreaking method for evaluating microbes through our Smart Probes™, a proprietary panel featuring 23 key microbes specifically identified as having the greatest impact on skin health. To further explore the intricate connection between acne and the skin microbiome, we employ qPCR technology. This cutting-edge technique allows us to precisely measure microbial populations on the skin, providing a clearer understanding of how imbalances in the microbiome can contribute to acne breakouts. By carefully analyzing how skincare products interact with both beneficial and acne-causing microbes, companies can create solutions that not only target acne symptoms but also support a healthier, balanced skin microbiome, promoting clearer and more resilient skin. Acne Panel Targets Cutibacterium acnes acnes (Subtype I) Cutibacterium acnes subtype I is a specific strain of the C. acnes bacterium commonly found within sebaceous (oil) glands. This bacterium plays a key role in maintaining skin health by breaking down sebum, and producing fatty acids that prevent harmful pathogens from colonizing. However, certain strains of subtype I are associated with inflammatory conditions like acne vulgaris. While typically harmless, its overgrowth or imbalance in the skin microbiome can trigger immune responses that lead to acne and other skin issues. Spittaels KJ, Ongena R, Zouboulis CC, Crabbé A, Coenye T. Cutibacterium acnes Phylotype I and II Strains Interact Differently With Human Skin Cells. Front Cell Infect Microbiol. 2020 Nov 16;10:575164. doi: 10.3389/fcimb.2020.575164. PMID: 33330124; PMCID: PMC7717938. Cutibacterium acnes defendens (Subtype II) Cutibacterium acnes subtype II is a strain of the C. acnes bacterium, distinct from subtype I, and is found on human skin, primarily in sebaceous areas. While it shares similarities with other subtypes in maintaining skin health by metabolizing sebum, subtype II is less commonly associated with acne development. It has been linked more to opportunistic infections and medical device-related infections rather than inflammatory acne. Subtype II is typically less inflammatory than subtype I and plays a different role in the skin's microbial balance. Cobian N, Garlet A, Hidalgo-Cantabrana C, Barrangou R. Comparative Genomic Analyses and CRISPR-Cas Characterization of Cutibacterium acnes Provide Insights Into Genetic Diversity and Typing Applications. Front Microbiol. 2021 Nov 3;12:758749. doi: 10.3389/fmicb.2021.758749. PMID: 34803983; PMCID: PMC8595920. Cutibacterium acnes elongatum (Subtype III) Cutibacterium acnes subtype III is a less common strain of the C. acnes bacterium, primarily found in deeper skin layers and associated more with opportunistic infections rather than acne. Unlike subtypes I and II, which are typically found on the skin's surface and sebaceous areas, subtype III is linked to post-surgical infections, particularly around medical implants, and is less involved in acne formation. It tends to have lower inflammatory potential, distinguishing it from other C. acnes subtypes in terms of both location and clinical relevance. Dagnelie MA, Khammari A, Dréno B, Corvec S. Cutibacterium acnes molecular typing: time to standardize the method. Clin Microbiol Infect. 2018 Nov;24(11):1149-1155. doi: 10.1016/j.cmi.2018.03.010. Epub 2018 Mar 12. PMID: 29544912. Industry Leader in Absolute Quantification Sequential’s Smart Probe ™ qPCR approach to microbiome analysis can resolve species and subspecies, giving statistically significant findings between skin conditions on in vivo human samples, compared to conventional sequencing approaches. Relevant Research Acne & The Skin Microbiome? Unveiling Nature's Acne Assassin: The Power of Essential Oils Benzoyl Peroxide & the Skin Microbiome Read More Articles