“heterogeneity” comes in two forms - biological (people have different skin microbiome) and technical (experimental/sample collection/processing), the above points are addressing both
Summarised more concisely here:
1. Targeted Demographics: We work closely with clients to clearly define the target demographic and skin type.
2. Strict Criteria: Inclusion and exclusion criteria minimize variability caused by treatments (e.g., antibiotics) or environmental differences (e.g., travel).
3. Smart Probes: These evaluate absolute levels of microbes on the skin, enabling us to measure exact quantities, track changes over time, and identify trends across clinical samples.
4. Synthetic Controls: Spike-in synthetic DNA controls manage variability during sample processing.
5. Standardized Protocols: Consistent, validated protocols are used across all labs, and we handle DNA extraction and qPCR in-house to ensure reliability.
6. Reproducible Sampling: Our skin patch collects samples from a consistent surface area for accurate and reproducible microbiome analysis.
Biological variability:
1. Targeted Demographics: We work closely with clients to clearly define the target demographic and skin type.
2. Strict Criteria: Inclusion and exclusion criteria minimize variability caused by treatments (e.g., antibiotics) or environmental differences (e.g., travel).
Technical variability:
1. Smart Probes: These evaluate absolute levels of microbes on the skin, enabling us to measure exact quantities, track changes over time, and identify trends across clinical samples.
2. Synthetic Controls: Spike-in synthetic DNA controls manage variability during sample processing.
3. Standardized Protocols: Consistent, validated protocols are used across all labs, and we handle DNA extraction and qPCR in-house to ensure reliability.
4. Reproducible Sampling: Our skin patch collects samples from a consistent surface area for accurate and reproducible microbiome analysis.