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in vivo Sampling is the Future of Microbiome Testing
We lead in clinical microbiome research and testing offering a comprehensive end-to-end platform designed to bring science-backed solutions to the personal care and pharma industry.
We offer an expansive platform to conduct research on personal care products through microbiome testing, biophysical assessments, and offer full recruitment services for studies. We are keen to publish our findings with our partners to increase the literature within this space.
At present our database of over 20,000 human microbiome samples is one of the most sophisticated within the industry and is growing rapidly.
Full End-To-End Testing Platform
in vivo Study Design
Collaborating closely with our clients to develop customised study protocols, ensuring that objectives are met with precision.
Validated Collection
Our non-invasive collection system consistently collects material for downstream analysis and allows for remote testing.
Analysis & Reporting
All analysis is carried out in-house allowing for a faster turn-around time on your study with exclusive access to our team of scientists and formulation experts.
White Paper Publication
Co-author a white paper with us based on the findings of your study to shorten the knowledge gap within the industry to demonstrate active's impact.
Candidate Recruitment
Carefully select human participants (location, age, etc.) to ensure the relevance and accuracy of the study.
Microbiome Detection
We offer four types of sequencing techniques including qPCR with our proprietary Smart Probes™, 16S, ITS and Shotgun Metagenomics.
Claims Certification
Providing comprehensive reports and certifications (Maintains the Microbiome seal) based on the study results which can be used in communication efforts.
Co-Presentation
We offer support by co-presenting at conferences where you want to demonstrate the impact of your study findings and explain the science behind the research.
State of the Art Sequencing
Using next-generation sequencing of the collection of micro-organisms found on the body, during product usage, Sequential investigates the microbial diversity, and particular micro-organisms we know are important and play a role in a healthy microbiome. With your pick from one of four sequencing reports, Sequential makes it easier to understand what depth of analysis is required for your particular needs.
qPCR Microbiome (Bacteria+Fungi)
Quantitative PCR (qPCR), also known as Real-Time PCR, is a method that Sequential uses in-house to measure the absolute number of copies of a microbial DNA defined by Sequential’s proprietary Smart Probes™. Using this method, we quantify the amount bacteria or fungi, allowing us to report level of species and strains within a given sample. The entire process is done at Sequential to ensure quality control, and extra control on your samples and privacy.
Next-Generation Sequencing of ITS (total fungal population)
ITS rRNA gene sequencing, or ITS amplicon sequencing is performed at Sequential to determine the relative abundance of taxa in a fungal community, and to compare between groups of interest. We know that fungi play a critical role in the microbiome of a healthy human, and it’s important to consider this application, especially for the scalp microbiome, and vaginal or vulva microbiome. We routinely offer two types of ITS rRNA primer regions: ITS1 & ITS2
Next-Generation Sequencing of 16S
16S rRNA gene sequencing, or 16S amplicon sequencing, is performed at Sequential to determine the relative abundance of taxa in a bacterial community, and to compare between groups of interest. This level of analysis can help to address changes in the overall bacterial profile over time or between treatment groups. We routinely offer two types of 16s rRNA primer regions: 16S V4 & 16S V1-V3
Shotgun Metagenomic Sequencing
Shotgun metagenome sequencing is performed at Sequential for taxonomic profiling (diversity and abundance), as well as functional analysis across different organisms in a more exploratory approach. This complex technique allows for parallel sequencing of DNA from all organisms (bacterial, fungal, viral and human) within the community, with good coverage for species-level detection. The data generation allows for more advanced reporting and genome assemblies.
